Obiettivo Scientific objectives and approach:The objective of this project is to develop an automated sensor that enables a quick, on-site assessment of genotoxicity to overcome the drawbacks of the current "comet assay". It will be based on the single cell gel (SCG) electrophoresis method, that is the so-called "comet assay", which is the best current technique for genotoxicity assessment.The innovation of this development is based on completely new equipment composed of:- The "multi-chamber wells" that is new tool enabling the on-site collection of samples and preventing the manipulation of probes during the test- An "automated carrier" that will support the multi-chamber wells and moves it under the microscope- A powerful software controlling the tray of the wells and interface to existing analysis software that will also be developed in the project.This development will enable the measure of up to 200-400 probes per day, instead of assessing 12-24 probes/1,5 day with the best current technique.Problems to be solved:Determination of the genotoxic potential of man-made chemicals (i.e. effluents, biozides, and chemicals accidentally released into the environment) is a subject of investigation all over the world. Methods measuring genotoxicity are time and cost consuming and need well-equipped cell culture laboratories as well as technical / scientific staff with experience in cell culture techniques.During the last 10 years, the comet assay (also named single cell gel electrophoresis assay (SCGE)) has been developed to a very sensitive assay for testing genotoxic properties of chemical and physical toxicants in mammalian cells.The disadvantages of the comet assay, as it is the case in all other modern genotoxicity tests lies in the small number of samples, which can be examined within one day. Highly specialized staff and a well-equipped cell culture laboratory are necessary to perform the assay.Expected Impacts:The impacts are:- increase of the number of samples/person and day- Reduction of required measuring time by 75% compared to existing methods (approximately 1 hour for 25 probes, instead of 12 probes a day)- Reduction of measuring costs by 50% compared to existing laboratory methods (automation of the sensor, then no need of high skilled trained personal in small companies to perform the test)- simplification of the procedure- enabling non-specialists to perform the assay- cell culture laboratory not necessary- greater competitiveness and profitability for the procedure of chemicals, biozides, effluents, by better control of genotoxicity. Campo scientifico natural sciencescomputer and information sciencessoftwarenatural sciencesphysical sciencesopticsmicroscopynatural sciencesphysical sciencesastronomyplanetary sciencescometsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsnatural scienceschemical scienceselectrochemistryelectrophoresis Programma(i) FP5-EESD - Programme for research, technological development and demonstration on "Energy, environment and sustainable development, 1998-2002" Argomento(i) 1.1.4.-1. - Key action Sustainable Management and Quality of Water Invito a presentare proposte Data not available Meccanismo di finanziamento CRS - Cooperative research contracts Coordinatore INTOX GMBH Contributo UE Nessun dato Indirizzo An den Eschen 43B 26129 OLDENBURG Germania Mostra sulla mappa Costo totale Nessun dato Partecipanti (8) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto EGI- AMBIENTE, PRESTACAO DE GESTAO E CONTROLE AMBIENTAL LDA Portogallo Contributo UE Nessun dato Indirizzo Praceta Madre de Deus 44 4405 VALANDARES Mostra sulla mappa Costo totale Nessun dato INSTITUTO TECNOLOGICO DE CASTILLA Y LEON Spagna Contributo UE Nessun dato Indirizzo C/ Lopez Bravo, 70 - Pol. Ind. Villalonquejar 09001 BURGOS Mostra sulla mappa Costo totale Nessun dato ISMAR CHIMICA SPA Italia Contributo UE Nessun dato Indirizzo Via Isocorte 16 16164 GENOVA Mostra sulla mappa Costo totale Nessun dato ISTITUTO NAZIONALE PER LO STUDIO E LA CURA DEI TUMORI Italia Contributo UE Nessun dato Indirizzo Via Mariano Semmola 80131 NAPOLI Mostra sulla mappa Costo totale Nessun dato MESUREX S.L. Spagna Contributo UE Nessun dato Indirizzo Pje. Pandereta, local 1 FUENGIROLA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato PARALAB-EQUIPAMENTOS INDUSTRIAIS E DE LABORATORIO, S.A. Portogallo Contributo UE Nessun dato Indirizzo Pç. Dos Pescadores 47 4450 333 MATOSINHOS Mostra sulla mappa Costo totale Nessun dato SOCIETA TECNOLOGIE BIOMEDICHE SRL Italia Contributo UE Nessun dato Indirizzo Viale delle acacie, 44 Edf A/5A 81031 AVERSA Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF OLDENBURG Germania Contributo UE Nessun dato Indirizzo Ammerlaender Heerstrasse 114/118 26111 OLDENBURG Mostra sulla mappa Costo totale Nessun dato