Cel Scientific objectives and approach:The objective of this project is to develop an automated sensor that enables a quick, on-site assessment of genotoxicity to overcome the drawbacks of the current "comet assay". It will be based on the single cell gel (SCG) electrophoresis method, that is the so-called "comet assay", which is the best current technique for genotoxicity assessment.The innovation of this development is based on completely new equipment composed of:- The "multi-chamber wells" that is new tool enabling the on-site collection of samples and preventing the manipulation of probes during the test- An "automated carrier" that will support the multi-chamber wells and moves it under the microscope- A powerful software controlling the tray of the wells and interface to existing analysis software that will also be developed in the project.This development will enable the measure of up to 200-400 probes per day, instead of assessing 12-24 probes/1,5 day with the best current technique.Problems to be solved:Determination of the genotoxic potential of man-made chemicals (i.e. effluents, biozides, and chemicals accidentally released into the environment) is a subject of investigation all over the world. Methods measuring genotoxicity are time and cost consuming and need well-equipped cell culture laboratories as well as technical / scientific staff with experience in cell culture techniques.During the last 10 years, the comet assay (also named single cell gel electrophoresis assay (SCGE)) has been developed to a very sensitive assay for testing genotoxic properties of chemical and physical toxicants in mammalian cells.The disadvantages of the comet assay, as it is the case in all other modern genotoxicity tests lies in the small number of samples, which can be examined within one day. Highly specialized staff and a well-equipped cell culture laboratory are necessary to perform the assay.Expected Impacts:The impacts are:- increase of the number of samples/person and day- Reduction of required measuring time by 75% compared to existing methods (approximately 1 hour for 25 probes, instead of 12 probes a day)- Reduction of measuring costs by 50% compared to existing laboratory methods (automation of the sensor, then no need of high skilled trained personal in small companies to perform the test)- simplification of the procedure- enabling non-specialists to perform the assay- cell culture laboratory not necessary- greater competitiveness and profitability for the procedure of chemicals, biozides, effluents, by better control of genotoxicity. Dziedzina nauki natural sciencescomputer and information sciencessoftwarenatural sciencesphysical sciencesopticsmicroscopynatural sciencesphysical sciencesastronomyplanetary sciencescometsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsnatural scienceschemical scienceselectrochemistryelectrophoresis Program(-y) FP5-EESD - Programme for research, technological development and demonstration on "Energy, environment and sustainable development, 1998-2002" Temat(-y) 1.1.4.-1. - Key action Sustainable Management and Quality of Water Zaproszenie do składania wniosków Data not available System finansowania CRS - Cooperative research contracts Koordynator INTOX GMBH Wkład UE Brak danych Adres An den Eschen 43B 26129 OLDENBURG Niemcy Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (8) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko EGI- AMBIENTE, PRESTACAO DE GESTAO E CONTROLE AMBIENTAL LDA Portugalia Wkład UE Brak danych Adres Praceta Madre de Deus 44 4405 VALANDARES Zobacz na mapie Koszt całkowity Brak danych INSTITUTO TECNOLOGICO DE CASTILLA Y LEON Hiszpania Wkład UE Brak danych Adres C/ Lopez Bravo, 70 - Pol. Ind. Villalonquejar 09001 BURGOS Zobacz na mapie Koszt całkowity Brak danych ISMAR CHIMICA SPA Włochy Wkład UE Brak danych Adres Via Isocorte 16 16164 GENOVA Zobacz na mapie Koszt całkowity Brak danych ISTITUTO NAZIONALE PER LO STUDIO E LA CURA DEI TUMORI Włochy Wkład UE Brak danych Adres Via Mariano Semmola 80131 NAPOLI Zobacz na mapie Koszt całkowity Brak danych MESUREX S.L. Hiszpania Wkład UE Brak danych Adres Pje. Pandereta, local 1 FUENGIROLA Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych PARALAB-EQUIPAMENTOS INDUSTRIAIS E DE LABORATORIO, S.A. Portugalia Wkład UE Brak danych Adres Pç. Dos Pescadores 47 4450 333 MATOSINHOS Zobacz na mapie Koszt całkowity Brak danych SOCIETA TECNOLOGIE BIOMEDICHE SRL Włochy Wkład UE Brak danych Adres Viale delle acacie, 44 Edf A/5A 81031 AVERSA Zobacz na mapie Koszt całkowity Brak danych UNIVERSITY OF OLDENBURG Niemcy Wkład UE Brak danych Adres Ammerlaender Heerstrasse 114/118 26111 OLDENBURG Zobacz na mapie Koszt całkowity Brak danych