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HIGH QUALITY OILS, PROTEINS AND BIOACTIVE PRODUCTS FOR FOOD AND NON-FOOD PURPOSES BASED ON BIOREFINING OF CRUCIFEROUS OILSEED CROPS

Exploitable results

Aqueous enzymatic extraction of cruciferous oilseed has been developed as a unique method for processing as a supplement to traditional extraction and processing methods. Thereby glucosinate degradation is avoided and the intact glucosinolates are isolated and evaluated as potential plant protection agents. In addition negative effects from these compounds in the protein, oil and lipid fractions have been avoided. The mild process preserves native antioxidants, which give the oil and protein fractions pronounced resistance towards autooxidation. The bioraf process thus produces a unique vegetable oil, with out residues of organic solvents or other comprehensive oil refining process. Protein concentrates, individual proteins, glucosinolates and phenolics are also products isolated from the process. Patent application has been submitted concerning production of high value proteins. Bioreactors with immobilized enzymes for the production of biocides have been constructed. Surplus products from the process are combined in a pellet suitable for cattle feeding. The process is thus without waste products, as all parts of the seed will be separated into usable products. Advanced but relatively simple and cheap methods of analyses as MECC, SFT, ELISA, and chemometrics are developed and used to efficient quality control of starting material, process, and end products. Exploitation of the results comprise: - Transfer of lab-scale developed techniques to the Pilot Plant scale. - Pilot Plant scale isolation of enzyme used in biocide production. - Pilot Plant scale isolation of glucosinolates as biocide precursors. - Production of added-value rapeseed oil for the use in feed, food, and non-food industry. - Production of protein concentrates and isolates for the use as special feed, food, and non-food. Opportunities for use of the biorefining techniques to: -Nature friendly Green Chemistry processing of oil and protein rich agricultural crops. - Production of high quality vegetable oil as a food with native antioxidants and with out organic solvent residues. - Production of added value proteins for food and special purpose with high quality demands. - Isolation of natural products for use as added value renewable fine chemicals; chiral, labile and structurally complex molecules. Combined results will give the needed basis for: - Increased value of agricultural crops. - Optimal utilization of renewable resources. - Benefits to producers, end users and the nature.

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