The pre-germination process (priming) has been studied with sugarbeet and tomato seeds particularly with regard to the solubilisation of seed storage proteins during the process. In our previous work on sugar beet seeds, we showed that priming induces the mobilisation of 11S globulin, resulting in the solubilisation in water of part of the globulin (its B subunit).
In this work, specific antibodies have been obtained for the 11S-globulin B-subunit of tomato seeds. Furthermore, a comparative study between this 11S-globulin solubilisation and the induction of cell cycle activity (as measured by flow cytometry of seed nuclei) during priming of tomato seeds, shows that the two phenomena are induced at different timing during priming, the globulin being first mobilised, followed by cell cycle reactivation.
Based upon use of antibodies against 11S-globulin B-subunit from tomato and sugarbeet, specific ELISAs have been designed and/or optimised. Such assays now allow readily following and optimising the priming treatments, without solely relying on germination assays. The ELISAs can also been used in single seed assays, allowing to characterise biochemical heterogeneity of commercial seed lots, in relation with their physiological heterogeneity (seed germination). Finally, the ELISAs can be used to assess the initial quality of commercial seed lots (certification analyses), i.e. to decipher whether they have been primed either intentionally or naturally (during seed development on the mother plant). Such assays can be used commercially.