Molecular analysis of g-protein coupled receptor kinases following manipulations of serotonergic and noradrenergic transmissions in the rat central nervous system

Informations projet

N° de convention de subvention: FMBI950509

Projet clôturé

Date de début 1 Octobre 1996

Date de fin 30 Septembre 1997

Financé au titre de

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Coût total € 0,00

Contribution de l’UE Aucune donnée

Coordonné par

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche
Italy

Objectif

Receptor desensitization is at the origin nf the progressive reduction of efficacy of some drug treatment. Adrenergic (beta2 and alpha2), muscarinic cholinergic, serotonergic (5-HT4) and many other G-protein coupled receptors (GR) are rapidly desensitized when stimulated by an agonist. G-protein coupled receptor kinases (GRK) and beta-arrestins are two families of proteins that are responsible for this process. Despite a better understanding of the molecular mechanisms involving
GRK/beta-arrestins little attention has been paid to the functional roles of these latter proteins.
The aim of our project is to investigates in some rat brain regions, the adaptative changes in GRK/beta-arrestins expression (mRNA and proteins) and activity followlng repeated antidepressant treatment (noradrenergic and serotonergic uptake inhibitor) or specific neural lesion (with 5,7-DHT or DSP-4 neurotoxins). In situ hybridization; Northern blot, rhodopsin phosphorylation assay and quantitative autoradiogaphy shall be used. GRK/,beta-arrestins-induced desensitization could become a new pharmacological targets.

Champ scientifique

• natural sciences › biological sciences › neurobiology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Thème(s)

• 0302 - Post-doctoral research training grants
• TL11 - Neurosciences (inc. Psychology)

Appel à propositions

Data not available

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche

• Contribution de l’UE: Aucune donnée
• Adresse: Via Nazionale ; 66030 Chieti - San Maria Imbaro ; Italie
• Coût total: Aucune donnée

Participants (1)

Not available

France

• Contribution de l’UE: Aucune donnée
• Coût total: Aucune donnée