Molecular analysis of g-protein coupled receptor kinases following manipulations of serotonergic and noradrenergic transmissions in the rat central nervous system

Informacje na temat projektu

Identyfikator umowy o grant: FMBI950509

Projekt został zamknięty

Data rozpoczęcia 1 Października 1996

Data zakończenia 30 Września 1997

Finansowanie w ramach

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Koszt całkowity € 0,00

Wkład UE Brak danych

Koordynowany przez

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche
Italy

Cel

Receptor desensitization is at the origin nf the progressive reduction of efficacy of some drug treatment. Adrenergic (beta2 and alpha2), muscarinic cholinergic, serotonergic (5-HT4) and many other G-protein coupled receptors (GR) are rapidly desensitized when stimulated by an agonist. G-protein coupled receptor kinases (GRK) and beta-arrestins are two families of proteins that are responsible for this process. Despite a better understanding of the molecular mechanisms involving
GRK/beta-arrestins little attention has been paid to the functional roles of these latter proteins.
The aim of our project is to investigates in some rat brain regions, the adaptative changes in GRK/beta-arrestins expression (mRNA and proteins) and activity followlng repeated antidepressant treatment (noradrenergic and serotonergic uptake inhibitor) or specific neural lesion (with 5,7-DHT or DSP-4 neurotoxins). In situ hybridization; Northern blot, rhodopsin phosphorylation assay and quantitative autoradiogaphy shall be used. GRK/,beta-arrestins-induced desensitization could become a new pharmacological targets.

Dziedzina nauki

• natural sciences › biological sciences › neurobiology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins

Program(-y)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Temat(-y)

• 0302 - Post-doctoral research training grants
• TL11 - Neurosciences (inc. Psychology)

Zaproszenie do składania wniosków

Data not available

System finansowania

RGI - Research grants (individual fellowships)

Koordynator

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche

• Wkład UE: Brak danych
• Adres: Via Nazionale ; 66030 Chieti - San Maria Imbaro ; Włochy
• Koszt całkowity: Brak danych

Uczestnicy (1)

Not available

Francja

• Wkład UE: Brak danych
• Koszt całkowity: Brak danych