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Contenido archivado el 2024-06-10

Biochemical characterization of the sec-independent bacteriocin transport system of lactococcus lactis and pediococcus acidilactici

Objetivo



Some strains of lactic acid bacteria secrete antimicrobial peptides which could be used as natural preservatives in food and feed fermentations. These so-called bacteriocins are secreted via a dedicated sec-independent secretion system consisting of (at least) two proteins. The aim of the project is to unravel the mechansim of bacteriocin secretion by determining the specificity of and the interaction between the two components of the transport system. By reciprocally exchanging the four proteins involved in the secretion of lactococcin A and pediocin PA- I, the specificity of both transport systems will be unravelled. By making protein fusions and by isolating antibodies against the proteins under study, the interaction between the proteins of each system will be analyzed. Various mutation/complementation strategies will further pinpoint the interacting domains.

Ámbito científico (EuroSciVoc)

Programa(s)

Tema(s)

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

RGI - Research grants (individual fellowships)

Coordinador

Rijksuniversiteit Groningen
Aportación de la UE
Sin datos
Dirección
30,Kerklaan
9751 NN Haren
Países Bajos
Coste total
Sin datos

Participantes (1)