Objectif Some strains of lactic acid bacteria secrete antimicrobial peptides which could be used as natural preservatives in food and feed fermentations. These so-called bacteriocins are secreted via a dedicated sec-independent secretion system consisting of (at least) two proteins. The aim of the project is to unravel the mechansim of bacteriocin secretion by determining the specificity of and the interaction between the two components of the transport system. By reciprocally exchanging the four proteins involved in the secretion of lactococcin A and pediocin PA- I, the specificity of both transport systems will be unravelled. By making protein fusions and by isolating antibodies against the proteins under study, the interaction between the proteins of each system will be analyzed. Various mutation/complementation strategies will further pinpoint the interacting domains. Champ scientifique natural scienceschemical sciencesorganic chemistryorganic acidsnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsmutationengineering and technologyindustrial biotechnologybioprocessing technologiesfermentation Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Thème(s) 0302 - Post-doctoral research training grants TL16 - Biotechnology Appel à propositions Data not available Régime de financement RGI - Research grants (individual fellowships) Coordinateur Rijksuniversiteit Groningen Contribution de l’UE Aucune donnée Adresse 30,Kerklaan 9751 NN Haren Pays-Bas Voir sur la carte Coût total Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Not available Italie Contribution de l’UE Aucune donnée Adresse Voir sur la carte Coût total Aucune donnée
