Immunocytochemical and biochemical characterization of fmrp in neurons

Información del proyecto

Identificador del acuerdo de subvención: FMBI982879

Proyecto cerrado

Fecha de inicio 1 Octubre 1998

Fecha de finalización 30 Septiembre 2000

Financiado con arreglo a

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Coste total Sin datos

Aportación de la UE Sin datos

Coordinado por

Erasmus Universiteit Rotterdam
Países Bajos

Objetivo

Research objectives and content
The fragile X syndrome is the most common form of inherited mental retardation in man and is caused by a dynamic mutation in the FMR-1 gene, that it is located in Xq27.3. The mutational basis of the syndrome is an expansion of a trinucleotide repeat (CGG) just upstream of coding region. As a result, transcription suppression through hypermethylation of the promoter region occurs. Lack of the expression of the gene product, FMRP, is responsible for the pathogenesis of the fragile X syndrome. Thus far the physiological funcion of FMRP is unknown. An understanding of the function of FMRP would provide insight into the molecular basis of the fragile X syndrome.The goal of this study is further characterization of FMRP in neurons using different experimental approaches.
Training content (objective, benefit and expected impact)
Using biotinylated antibodies against FMRP I will study the expression pattern of FMRP in normal and transgenic mice, with special emphasis to the central nervous system. At the subcellular level I will study the routing of FMRP, using cultured neurons which are transfected with a plasmid containing either wildtype or mutant forms of FMRP.In addition, biochemical studies will be performed to characterize tne different molecular forms and possible interaction(s) of FMRP with other proteins or RNA's. Links with industry / industrial relevance (22)

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural. Véase: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• ciencias naturales › ciencias biológicas › neurobiología
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas
• ciencias naturales › ciencias biológicas › genética › mutación
• ciencias naturales › ciencias biológicas › genética › ARN

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Programa(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Tema(s)

• 0302 - Post-doctoral research training grants
• TL05 - Cell Biology

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

RGI -

Coordinador

Erasmus Universiteit Rotterdam

• Aportación de la UE: Sin datos
• Dirección: 50,Dr. Molewaterplein ; 3015 GE Rotterdam ; Países Bajos
• Coste total: Sin datos