Folding and stability studies of the SH3 and Gb domains

Informazioni relative al progetto

ID dell’accordo di sovvenzione: INTAS-93-0007

Progetto chiuso

Data di avvio 1 Maggio 1995

Data di completamento 30 Aprile 1996

Finanziato da

• International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Costo totale

Nessun dato

Contributo UE

€ 42 000,00

Coordinato da

Universidad de Granada
Spain

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Risultati sfruttabili

Folding and stability studies of the SH3 and Gb domains

The spectrin SH3-fragment is an excellent model for investigating the energetics of minimal globular domain folding. The participants in this project will purify and study the naturally occurring variants of the SH3 domains and design some of their mutants with a view to changing fragment stability. Special attention will be paid to the mutants with variable lengths in the loops connecting the elements of secondary structure and to chimeras of the SH3. This project also includes studies of the interaction between SH3 domains and synthetic oligopeptides modelling the structure of the functionally important recognition partners of the domain. A multidisciplinary thermodynamic, kinetic and structural approach will be used to study folding/unfolding patterns of these species and to characterize their stability. The Russian group will purify proteins and carry out the differential scanning calorimetry and optical studies. One of the members of this group will be directly involved in mutant engineering and protein-peptide modelling studies by joining the German group.