In the last decade an increasing interest of researchers from both fundamental and applied domains has been focused on studying protein stability and enzyme regulation under extreme conditions. Together with the attempts at better understanding the proteins from extremophiles (thermophiles, halophiles, barophiles etc.), extreme conditions are used for the enzymes from mesophilic sources.
The goal of this project was two-fold: determine structural principles lying at the basis of function and stability of proteins and more complex systems; and adapt the enzymes to extreme conditions of high temperatures, high pressures, and organic solvents.
This project includes five major research topics and co-ordination activity:
- Baroenzymology in reversed micelles,
- Electrophorensis of proteins in reversed micelles,
- Pressure effects on protein stability,
- Additivity in effects of extreme conditions on proteins,
- Enzymes in cyro- and barogels.