Objective
The efficiency of DNA damage response is a subject of normal populational variations. The reduced ability to combat DNA alterations is associated with cancer susceptibility and can be attributed either to deficient DNA repair or, alternatively, to suboptimal cell death capacity. While the relationships between cancer risk and polymorphism of DNA repair genes are being intensively studied, the alternative aspect of the DNA damage response, i.e. apoptosis, has been unjustly overlooked in the research of tumour-associated gene polymorphisms.
This project is aimed at testing the hypothesis whether single nucleotide polymorphisms (SNPs) associated with the reduced apoptotic capacity contribute to the increased cancer risk. The following activities will be carried out:
1) Identification and validation of functional and tagging SNPs in apoptosis genes:
a) Identification of already known SNPs in research publications and Internet databases;
b) Validation of the occurrence of 'dubious' SNP by real-time AS-PCR genotyping;
c) Search for the new SNPs in previously non-tested genes by direct DNA sequencing.
2) Study on the role of apoptosis-related SNPs in cancer predisposition:
a) Preliminary study of the involvement of SNP candidates in breast cancer (BC) and lung cancer (LC) susceptibility: 'comparison of extremes' approach;
b) Traditionally designed, large case-control study for the promising SNP candidates. 3) Study on the functional significance of SNPs in apoptotic genes: analysis of correlation between individual genotype and the extent of apoptosis in lymphocytes exposed to DNA damaging dose of ionizing radiation.
4) Comparison of the rate of DNA damage induced apoptosis in lymphocytes isolated from cancer patients vs. healthy controls.
5) Comparison of radiation-induced transcriptional activation of apoptotic genes in 'high' vs. 'poor' apoptotic responders using cDNA array profiling and RT-PCR analysis.
If successful, the project will significantly improve the fundamental understanding of mechanisms of cancer susceptibility as well as facilitate the development of practical approaches for the detection of at-risk individuals.
Fields of science (EuroSciVoc)
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
LEIDEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.