Development of novel gene expression and genome modification strategies

Objective

Gene transfer protocols used for bio molecule productions and therapeutic strategies suffer from lack of control of the fate of the foreign DNA within the host genome. The efficient expression of the transferred DNA relies on the site of its integration, on the presence of cis DNA regulatory elements and on nuclear proteins which participate in the formation of the chromatin at the transgeniclocus. This proposal aims at studying all these contributing factors in order to develop methods which can target the foreign DNA at preselected, precharacterised sites in the genome and guarantee stable and/or regulatable expression of the transferred genes, without interrupting or activating endogenous neighbouring gene loci.

Fields of science

• medical and health sciences › medical biotechnology › genetic engineering › gene therapy
• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › genetics › genomes

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-8. - Research into genomes and diseases of genetic origin

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

MRC National Institute for Medical Research

Address

The Ridgeway Mill Hil
NW7 1AA London
United Kingdom

Participants (7)

ERASMUS UNIVERSITEIT ROTTERDAM

Netherlands

Address

Dr. Molewaterplein 50
3000 DR Rotterdam

FOUNDATION OF RESEARCH AND TECHNOLOGY - HELLAS

Greece

Address

Vassilika Vouton
71110 Iraklion

GBF - NATIONAL CENTRE FOR BIOTECHNOLOGY

Germany

Address

1,mascheroder Weg 1
38124 Braunschweig

IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE

United Kingdom

Address

Du Cane Road
W12 0NN London

ROSLIN INSTITUTE (EDINBURGH)

United Kingdom

Address

EH25 9PS Roslin, Midlothian

RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG

Germany

Address

Im Neuenheimer Feld 282
69120 Heidelberg

UNIVERSITÀ DEGLI STUDI DI ROMA TOR VERGATA

Italy

Address

135,via Di Tor Vergata 135
00133 Roma