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IMPROVEMENTS IN THE DETERMINATION OF FOLATES IN FOOD

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Comparisons of the performance of the different types of method for analysis of folate in food has been carried out with a view to identifying improved and more specific procedures. This showed the need to improve extraction and deconjugation procedures, calibration and identification of folate forms by high performance liquid chromatography (HPLC) and calibration in the PBA/RIA (protein binding assay/radioimmunoassay) methods.
The current project is undertaken as a consequence of an intercomparison made under project no 341 which identified serious difficulties in the analysis of folate in food.

The objective is to examine through intercomparisons the performance of the different types of method with a view to identifying improved and more specific procedures for folate possibly based on HPLC or immunoassays which can offer potentially greater sensitivity. The folate content of several food RMs will be certified if it is feasible.

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Laboratory of the Government Chemist (LGC)
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