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Contenuto archiviato il 2024-04-16

A collaborative study on the nature and pathogenesis of exfoliation glaucoma

Obiettivo


One part of the project determined the proteins present in the matrix of exfoliation substance and the sites where exfoliation substance is synthesised. In all specimens, amyloid P component and an elastin microfibrillar associated glycoprotein, gp115 was identified in exfoliation substance. Elastin was present in the stroma of the normal iris and of the exfoliative iris but not within exfoliation substance. The most likely source of exfoliation substance within the stroma of the iris seemed to be the perivascular cells which surround the vascular endothelium, and the vascular endothelial cells were not primarily involved.

The most likely source of exfoliation substance within the stroma of the iris seemed to be the perivascular cells which surround the vascular endothelium, and the vascular endothelial cells were not primarily involved.

A prospective consecutive study has been set up to establish the prevalence of exfoliation syndrome and its influence on the surgical outcome in cataract surgery.

Another study was set up to determine the terminal glycosylation of structural glycoproteins supposed to form a constituent part of exfoliation substance, using lectin histochemistry and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) with blotting and lectin binding. Formalin fixation of exfoliation material induced conformational changes in the glycoproteins in such a way that various sugar moieties were presented in random fashion to the lectins. Unfixed exfoliation material consistently presented the same sugar moieties and harboured a region with a cluster of fucose molecules, which was not expressed in normal basement membranes. However, both exfoliation material and basement membranes expressed fucose in single molecular arrangements. Boiling with SDS uncoiled the glycoproteins exposing fucose in both normal and exfoliative substances.
Some of these apparently contradictory findings could be explained by the following hypothesis. In the disease state there is an exfoliation glycoprotein, which partially resembles a structural glycoprotein normally present in the basement membrane complex, but which is not, however, in a fully glycosylated form. If one supposes that the normal fully glycosylated molecule is paired or mirror imaged and curled up in such a fashion that more centrally located fucose molecules are masked, these regions will not be accessible to the tissue plasminogen activator (TPA) lectins. In the abnormal state, a lack of terminal sugar moieties on one of the glycoprotein pairs may expose the fucose molecules, thus making them accessible to the TPA lectin.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP. Cfr.: Il Vocabolario Scientifico Europeo.

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Coordinatore

University of Glasgow
Contributo UE
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Indirizzo
38 Church Street
G11 6NT Glasgow
Regno Unito

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