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CHLOROPHYLL FLUORESCENCE AS A POSSIBLE TOOL FOR LOW-COST AND CONTINUOUS BIO-MONITORING OF PESTICIDES IN AQUATIC ECOSYSTEMS

Ziel

The objective is to develop low cost techniques which allow continuous and sensitive monitoring of a wide range of pesticides in all aquatic ecosystems.

It is intended to develop the physiological and conceptional basis for a biosensor system which allows detection of low level changes in pesticide concentrations in natural habitats. In order to use the in vivo chlorophyll fluorescence as a test parameter for environmental control, the different parameters which contribute to steady state fluorescence level must be clearly defined.

The programme includes the effect of preillumination, light intensity and light quality on the fluorescence levels in 4 different algal species, 2 fresh water and 2 marine. The inhibition constants of 4 selected herbicides are determined for all the fluorescence parameters. The methodology used is pulse amplitude modulated fluorescence of intact algal cells under different light conditions with the following pesticides: diuron, simazine, atrazine and metazine.

After a screening phase, a candidate system is chosen according to the criteria of sensitivity, reproducibility and stability of culture maintenance.

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Johannes-Gutenberg-Universität Mainz
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