Dissecting dsRNA uptake in RNAi-based antiviral immunity

Projektinformationen

DDRR

ID Finanzhilfevereinbarung: 656398

DOI 10.3030/656398

Projekt abgeschlossen

EK-Unterschriftsdatum 30 April 2015

Startdatum 1 Juni 2015

Enddatum 31 Mai 2017

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 185 076,00

EU-Beitrag € 185 076,00

Koordiniert durch

INSTITUT PASTEUR
France

Ziel

Important viral infectious diseases, such as dengue and chikungunya, are transmitted to humans by insect
vectors. One of the key factors that modulates whether an insect is competent or not to transmit a given
pathogen is its innate immune response. The major antiviral defense in insects is the RNA interference
(RNAi) mechanism that is activated by the detection of viral double-stranded RNA (dsRNA). During
antiviral RNAi a silencing immune signal is transported from one cell to another to set an antiviral state
(systemic RNAi). To be primed, non-infected cells must sense this silencing signal and internalize it.
Although dsRNA as a mediator of local antiviral immunity is well established in insects, the effectors of a
systemic immune response are not identified. For instance, the identification of the dsRNA receptor in
insects remains elusive. This proposal intends to dissect the mechanism of dsRNA uptake in insects with
special focus on discovering its receptor. I propose to combine functional (genome wide RNAi screen, in
vivo dsRNA oral uptake) with binding (electrophoretic mobility shift assay, surface plasmon resonance) and
structural assays (expression, production and studies of complexes receptor/dsRNA) using Drosophila
melanogaster as insect model and an array of viruses in order to identify and characterize the dsRNA
receptor.To be found, the manipulation of this receptor could help control the insect vectors of emerging
viral diseases. Understanding how the infection is controlled within the insect before crossover to the human
host could generate new strategies to disrupt pathogen transmission.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › microbiology › virology
• medical and health sciences › health sciences › infectious diseases
• medical and health sciences › basic medicine › immunology
• natural sciences › biological sciences › zoology › entomology
• medical and health sciences › basic medicine › pharmacology and pharmacy › pharmaceutical drugs › antivirals

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2014

Finanzierungsplan

MSCA-IF-EF-ST - Standard EF

Koordinator

INSTITUT PASTEUR

• Netto-EU-Beitrag: € 185 076,00
• Adresse: RUE DU DOCTEUR ROUX 25-28 ; 75724 Paris ; Frankreich
• Region: Ile-de-France › Ile-de-France › Paris
• Aktivitätstyp: Research Organisations
• Gesamtkosten: € 185 076,00