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Carbohydrate-based fine chemicals: Development of a glycosylation platform cell factory and optimization of downstream processing for the sustainable production of glycosides.

Deliverables

First data management plan

Data management plan, periodically updated

Second data management plan

Second data management plan, periodically updated

Installation of Stakeholder advisory board

Installation of Stakeholder advisory board

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Publications

Plasmid Design for Tunable Two‐Enzyme Co‐Expression Promotes Whole‐Cell Production of Cellobiose

Author(s): Schwaiger, Katharina N.; Voit, Alena; Dobiasová, Hana; Luley-Goedl, Christiane; Wiltschi, Birgit; Nidetzky, Bernd
Published in: Biotechnology Journal, 2020, ISSN 1860-7314
DOI: 10.1002/biot.202000063

Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria

Author(s): Chao Zhong, Christina Ukowitz, Konrad J. Domig, Bernd Nidetzky
Published in: Journal of Agricultural and Food Chemistry, Issue 68/32, 2020, Page(s) 8557-8567, ISSN 0021-8561
DOI: 10.1021/acs.jafc.0c02660

On the donor substrate dependence of group‐transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase‐catalyzed transglycosylation

Author(s): Mario Klimacek, Alexander Sigg, Bernd Nidetzky
Published in: Biotechnology and Bioengineering, Issue 117/10, 2020, Page(s) 2933-2943, ISSN 0006-3592
DOI: 10.1002/bit.27471

Engineering of cellobiose phosphorylase for the defined synthesis of cellotriose

Author(s): Zorica Ubiparip, David Sáez Moreno, Koen Beerens, Tom Desmet
Published in: Applied Microbiology and Biotechnology, Issue 104/19, 2020, Page(s) 8327-8337, ISSN 0175-7598
DOI: 10.1007/s00253-020-10820-8

Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

Author(s): Chao Zhong, Christiane Luley‐Goedl, Bernd Nidetzky
Published in: Biotechnology and Bioengineering, Issue 116(9), 2019, Page(s) 2146-2155, ISSN 0006-3592
DOI: 10.1002/bit.27008

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Author(s): Jorick Franceus, Tom Desmet
Published in: International Journal of Molecular Sciences, Issue 21/7, 2020, Page(s) 2526, ISSN 1422-0067
DOI: 10.3390/ijms21072526

Three‐Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello−oligosaccharides

Author(s): Chao Zhong, Božidar Duić, Juan M. Bolivar, Bernd Nidetzky
Published in: ChemCatChem, Issue 12/5, 2020, Page(s) 1350-1358, ISSN 1867-3880
DOI: 10.1002/cctc.201901964

Downstream processing technologies in the biocatalytic production of oligosaccharides

Author(s): Andreas Kruschitz, Bernd Nidetzky
Published in: Biotechnology Advances, Issue 43, 2020, Page(s) 107568, ISSN 0734-9750
DOI: 10.1016/j.biotechadv.2020.107568

Removal of glycerol from enzymatically produced 2-α-d-glucosyl-glycerol by discontinuous diafiltration

Author(s): Andreas Kruschitz, Bernd Nidetzky
Published in: Separation and Purification Technology, Issue 241, 2020, Page(s) 116749, ISSN 1383-5866
DOI: 10.1016/j.seppur.2020.116749

Three‐Enzyme Phosphorylase Cascade for Integrated Production of Short‐Chain Cellodextrins

Author(s): Chao Zhong, Bernd Nidetzky
Published in: Biotechnology Journal, Issue 15/3, 2020, Page(s) 1900349, ISSN 1860-6768
DOI: 10.1002/biot.201900349

Reactive extraction of fructose for efficient separation of sucrose-derived glucosides produced by enzymatic glycosylation

Author(s): Kruschitz, Andreas; Nidetzky, Bernd
Published in: Green Chemistry, Issue 22(15), 2020, ISSN 1463-9270

Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials

Author(s): Bernd Nidetzky, Chao Zhong
Published in: Biotechnology Advances, 2020, Page(s) 107633, ISSN 0734-9750
DOI: 10.1016/j.biotechadv.2020.107633