Transferring autonomous and non autonomous cell degeneration 3D models between EU and USA for development of effective therapies for neurodegenerative diseases (ND)

Project Information

CROSS-NEUROD

Grant agreement ID: 778003

DOI 10.3030/778003

Project closed

EC signature date 8 February 2018

Start date 1 June 2018

End date 31 March 2024

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 693 000,00

EU contribution € 594 000,00

Coordinated by

UNIVERSITA DEGLI STUDI DI MILANO
Italy

Deliverables

Websites, patent fillings, videos etc. (5)

Report for the on-site training provided and usage of the online system every 6 months

We will report for the onsite training provided and usage of the online system

Evaluation of neuroprotective abilities of candidate genes in an in vitro model.

We will assess gene expression profile and identify candidate causal MRs and shared molecular pathways between NDs.

3D models generated from 4 different sources

Patient specific minibrain and spinal cordorganoids generated from iPSCs of ALS SMA FTD and PD will be characterized

Pre-clinical protocols for AAV gene delivery

We will establish protocols to over-express or knock-down genes of interest by using adeno-associated virus (AAV).

Identification of neuropathological markers

We will identify genes responsible for selective cell death and potential targets to modify ND disease progression

Publications

Conference proceedings (1)

Human 3D cell culture systems to model and elucidate spinal muscular atrophy pathology and treatment

Author(s): *S. CORTI1, I. FARAVELLI1, P. RINCHETTI1, S. MANCINELLI2, L. MAPELLI3, G. FOROTTI4, M. RIZZUTI4, C. CORDIGLIERI5, L. CALANDRIELLO4, S. TAMANINI1, N. BRESOLIN1, G. P. COMI1, S. LODATO2, M. NIZZARDO1
Published in: 2019
Publisher: SFN

Peer reviewed articles (3)

CSF transplantation of a specific iPSC-derived neural stem cell subpopulation ameliorates the disease phenotype in a mouse model of spinal muscular atrophy with respiratory distress type 1.

Author(s): Forotti G1, Nizzardo M1, Bucchia M2, Ramirez A2, Trombetta E3, Gatti S4, Bresolin N5, Comi GP5, Corti S6.
Published in: Experimental Neurology, 2019, ISSN 0014-4886
Publisher: Academic Press
DOI: 10.1016/j.expneurol.2019.113041

iPSCs-Based Neural 3D Systems: A Multidimensional Approach for Disease Modeling and Drug Discovery

Author(s): Gianluca Costamagna, Luca Andreoli, Stefania Corti, Irene Faravelli
Published in: Cells, Issue 8/11, 2019, Page(s) 1438, ISSN 2073-4409
Publisher: Multidisciplinary Digital Publishing Institute
DOI: 10.3390/cells8111438

Disease Modeling and Therapeutic Strategies in CMT2A: State of the Art

Author(s): Kordelia Barbullushi, Elena Abati, Federica Rizzo, Nereo Bresolin, Giacomo P. Comi, Stefania Corti
Published in: Molecular Neurobiology, Issue 56/9, 2019, Page(s) 6460-6471, ISSN 0893-7648
Publisher: Humana Press, Inc.
DOI: 10.1007/s12035-019-1533-2