Project description
Dissecting the mechanism of mRNA surveillance
Eukaryotic cells have evolved a complex mechanism to regulate the flow of information from gene to protein. At the mRNA level, there is a surveillance mechanism in place, the nonsense-mediated mRNA decay (NMD) pathway, which detects faulty mRNAs and degrades them. Scientists of the EU-funded UPFs_NMD project propose a multidisciplinary approach to study the molecular architecture and interaction between the different NMD components. Results will provide important insight into the structure and function of the NMD machinery and help delineate its implication in various diseases, including neurological disorders.
Objective
In eukaryotes, gene expression is highly regulated involving multistep pathways in which mRNA plays a crucial role. Cells have evolved surveillance mechanisms able to detect and degrade defective transcripts. Nonsense-mediated mRNA decay (NMD) is a mRNA surveillance mechanism which detects faulty mRNAs with premature termination codons (PTCs) and targets these transcripts to decay. By modulating the expression of physiological mRNAs, NMD acts as a post-transcriptional regulator controlling important cellular processes in development, stress response, immunity and neuronal differentiation. NMD is of medical importance because mutations or copy number variations of the NMD factors are implicated in human neurological disorders, intellectual disability, schizophrenia, autism, immune diseases and cancer. The mammalian NMD machinery comprises the proteins UPF1, UPF2, and UPF3B, eukaryotic release factors (eRF1 and eRF3a), SMG1 kinase and SMG5-9. Recently UPF3B was found to have a role in translation termination at a premature stop codon, interacting directly with the ribosome, release factors and UPF1, requiring modification of prevalent NMD models. To understand the molecular mechanisms of UPF3B and its role in NMD, this proposal aims to determine the molecular architecture of UPF3B in complex with UPF1, ribosome and mRNA. Furthermore, I will explore the role of the helicase and ubiquitin ligase activities of UPF1 in NMD and protein decay. I will use biochemistry, biophysics, X-ray crystallography and cryo-electron microscopy to identify the UPF3B domains involved in the recognition of NMD substrates and to investigate how UPF3B binding to the UPF1-RNA complex helps trigger mRNA decay.
Such information will shed light on how translation termination and assembly of the NMD machinery are coordinated and therefore will be of key importance for the future development of therapeutic approaches for the future development of therapeutic approaches for NMD- related diseases.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences biophysics
- medical and health sciences clinical medicine psychiatry schizophrenia
- medical and health sciences basic medicine neurology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
BS8 1QU BRISTOL
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.