Project description
Decoding gene regulation
Gene enhancers are important in regulating spatio-temporal gene expression, essential for tissue differentiation and cell-type diversification. However, how these enhancers identify and interact with their target gene promoters remains poorly understood. Supported by the Marie Skłodowska-Curie Actions programme, the DEPI project seeks to unravel this mystery using Nuclear-Titrated (NuTi) Capture-C, a genome-wide technique that maps enhancer-promoter interactions (EPIs) at high resolution. By analysing a vast dataset of over 16 000 gene promoters across multiple developmental stages of Drosophila embryos, researchers aim to build an atlas of tissue- and stage-specific EPIs. Integrating genomic datasets, the project will uncover features driving E-P specificity and identify factors regulating these interactions, creating a web-based resource for the broader genome regulation community.
Objective
Enhancers control spatio-temporal gene expression, which is central to the diversification of cell-types and tissue differentiation. Enhancers can be located in close proximity to, or distally from, their target genes promoters, including in introns of non-cognate genes. To regulate gene expression, distal enhancers (E) must come into proximity to interact with their promoters (P) through folding/looping of the intervening DNA. How enhancers identify and regulate their specific target gene remains poorly understood and is subject of this proposal. Chromosome conformation capture based techniques such as Nuclear-Titrated (NuTi) Capture-C are powerful tools for the genome-wide investigation of enhancerpromoter proximity/interactions (EPIs) at sub-kilobase resolution. This project aims to uncover general features of EPIs by measuring and characterizing EPIs using NuTi Capture-C across many different developmental stages and tissues during Drosophila embryogenesis. I will analyze a massive Capture-C dataset already generated by host lab, capturing interactions from 16,663 genes promoters each at eight different conditions (with four replicates), thereby providing unprecedented scale and resolution. I will integrate this Capture-C resource with multiple genome-wide datasets (gene expression, histone marks and chromatin accessibility) from the host lab and others. The analysis of this comprehensive EPIs atlas will link enhancers to promoters (genome-wide), characterize features driving E-P specificity and potentially identify novel factors involved in their establishment. This new view of tissue- and developmental stage- specific EPIs will serve as a unique resource that we will share through a user-friendly web-based portal to a large community using this important model organism. The biological insights that we uncover, including features linked to E-P specificity and tissues-specific regulation, will interest a very broad community studying genome regulation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences developmental biology
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
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Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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69117 Heidelberg
Germany
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