Obiettivo The objective of the project is the development of cost-efficient and environmental sound recovery and conversion processes for production of new high quality products from biorefined cruciferous oilseed crops.The target is separation, isolation and production of new high quality products from the valuable oils, proteins and various types of other biomolecules occurring in cruciferous crops, especially rapeseed, for new food and non-food markets as supplement to and replacement of environmental incriminating products.The project is based on a new method of processing cruciferous oilseed using an aqueous enzymatic degradation of cell walls followed by separation in decanters and centrifuges. The process does not include use of organic solvents, but a special Flash Chromatography (FC) technique is applied for the isolation of glucosinolates and lipophilic rapeseed proteins; in a complex with amphiphilic lipids (LIPRO) they are isolated by a simple sieving step. Water used in the enzymatic reaction is reused for saving water and increasing the yield of oil, and water removed by drying of the products is replaced by tap-water. An initial step is inactivation of the seed enzymes, myrosinases, lipases and lipoxygenases, protecting the native biomolecules, glucosinolates, oils and natural antioxidants against transformation into artefacts.The process, which is environmentally friendly and a gentle oilseed processing with separations occurring in an aqueous emulsion results in the six fractions; oils, protein rich meal (PRM), lipophilic proteins in a complex with amphiphilic lipids (LIPRO), hydrophillic-amphiphilic compounds (HAC), glucosinolates and hull-fibres. This is thus a unique process, where problems caused by glucosinolate products are eliminated, and the oils do not need degumming as phospholipids are extracted into the emulsion. Residues of organic solvents do not create problems and as the fractions PRM, LIPRO and HAC do not contain hulls, it gives a unique starting point for isolation and production of high quality products of the rapeseed storage proteins, cruciferin, napin and oleosins, as well as amphiphilic compounds as phospholipids, dolichols and natural antioxidants. The project will be concentrated on production of new non-food and food/feed products from both oils and proteins with and without modification of the proteins, application of the products including the hydrophillic-amphiphilic compounds for production of emulsifiers, surfactants and natural antioxidants. A special technique with membranes containing immobilised enzymes (myrosinases) will be used for transformation of glucosinolates isolated by FC into products which will be tested as biocides or biodegradable natural pesticides.The projects comprises an Inter European cooperation including Italy, Germany, France, Scotland and Denmark, which covers a coordinated multi- disciplinary proposal and a wide spectrum of unutilized opportunities for obtaining high added value 'Green-products' from the cruciferous oilseeds. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculeslipidsagricultural sciencesagriculture, forestry, and fisheriesagriculturegrains and oilseedsoilseedsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programma(i) FP4-FAIR - Specific research, technological development and demonstration programme in the field of agriculture and fisheries (including agro-industry, food technologies, forestry, aquaculture and rural development), 1994-1998 Argomento(i) 1.2.2 - Processing of raw materials Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore THE ROYAL VETERINARY AND AGRICULTURAL UNIVERSITY Contributo UE Nessun dato Indirizzo THORVALDSENSVEJ, 40 1871 FREDERIKSBERG C Danimarca Mostra sulla mappa Costo totale Nessun dato Partecipanti (5) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CASTROL A/S Danimarca Contributo UE Nessun dato Indirizzo AMAGER STRANDVEJ, 162 2300 COPENHAGEN S Mostra sulla mappa Costo totale Nessun dato FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V. Germania Contributo UE Nessun dato Indirizzo STEINERSTRAßE, 15 81369 MÜNCHEN Mostra sulla mappa Costo totale Nessun dato I.N.R.A. Francia Contributo UE Nessun dato Indirizzo RUE DE LA GÉRAUDIÈRE - P.O. BOX 1627 44316 NANTES Mostra sulla mappa Costo totale Nessun dato Istituto Sperimentale per le Colture Industriali Italia Contributo UE Nessun dato Indirizzo Via di Corticella 133 40129 Bologna Mostra sulla mappa Costo totale Nessun dato Scottish Agricultural College (SAC) Regno Unito Contributo UE Nessun dato Indirizzo 581 King Street AB9 1UD Aberdeen Mostra sulla mappa Costo totale Nessun dato
