Objective
The immune response is a potent defensive reaction within the organism that can lead to deleterious self-damaging effects in absence of control mechanisms. The modulation of the immune response is exerted at many different levels including T cell-mediated immuno-regulation. Regulatory T cells (TR cells) have recently been identified which have the capacity to control the activity of effectors T cells. Operationally, TR cells are defined by the ability to suppress the expansion of pathogenic naive T cells in vitro and to inhibit inflammatory reactions in mouse experimental systems. However, the mechanisms by which TR cells mediate immune-regulatory events are poorly described. In fact, it is not known whether this TR cell mediated suppression requires a direct cell-to-cell contact between TR cells, Antigen Presenting Cells (APCs) and target Tcells. Furthermore, the stochiometry of the cellular interactions, the dynamics of such interactions and the site(s) of in vivoimmune regulation are largely unknown. The aim of this project is to characterise the cellular interactions occurring during immune regulation events in vitro using the suppression assay system, and in vivo by analysing intact organs of different animal models of immune regulation. Conventional and multi-photon confocal microscopy will be applied to visualise invitro and in vivo interactions of APCs with CD4+CD25+ TR cells and naïve (effector) T cells previously sorted by flowcytometry, from Yellow Fluorescent Protein and Cyan Fluorescent Protein transgenic mice, respectively. These experiments will be designed to assess (1) whether CD4+CD25+ TR cells suppress naïve T cells by direct interaction or through an indirect effect via APCs, (2) whether the CD4+CD25+ TR cell-mediated immune regulation occurs at the site of tissue inflammation or in draining lymph nodes and (3) whether the kinetics of cell migration to the sites of immune regulation and tissue inflammation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine immunology immunisation
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy confocal microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LISBOA
Portugal
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.