Obiettivo The objective of this proposal comes from a wish and need in fluorescence-based bioanalytical studies to detect single molecules, one by one, even at high biological concentrations.At present, commonly used optical microscopes applied in the detection of single molecules, using diffraction-limited observation volumes (lateral size ~200nm; axial ~ 500 nm), only allow for single molecules to be detected at pico- to nanomolar concentrations. The aim of the proposal is to develop a new tool that makes spectroscopic measurements possible even at higher concentrations, i.e. in the micromolar range.The novel tool will combine STED (stimulated emission depletion) and FCS (fluorescence correlation spectroscopy) microscopy. The multidisciplinary STED-FCS project intends to generate a non-contact optical technique, where a detection volume can be placed anywhere in solution, including inside of cells. This will open the possibility to study single biomolecules in ultra-small focal volumes, even at high concentrations, in a non-invasive fashion.The fellowship will make it possible for the applicant to acquire very unique skills and give him the opportunity to develop and use a new state-of-the-art nanospectroscopic tool. Moreover, this will definitively secure his future academic career in the area of ultra-sensitive fluorescence spectroscopy. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesnatural sciencesphysical sciencesopticsspectroscopyemission spectroscopynatural sciencesphysical sciencesopticsmicroscopysuper resolution microscopy Parole chiave Stimulated emission depletion (STED) fluorescence correlation spectroscopy (FCS) single molecule detection (SMD) Programma(i) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Argomento(i) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Invito a presentare proposte FP6-2002-MOBILITY-5 Vedi altri progetti per questo bando Meccanismo di finanziamento EIF - Marie Curie actions-Intra-European Fellowships Coordinatore MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Contributo UE Nessun dato Indirizzo Hofgartenstrasse 8 MUENCHEN Germania Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato
