Descrizione del progetto
Gli esosomi artificiali svelano aspetti importanti della comunicazione dell’RNA
L’ingegneria dei sistemi viventi che simula processi biologici importanti rappresenta una sfida di primaria importanza. Allo stesso tempo, i sistemi sintetici offrono uno strumento potente per studiare i percorsi di comunicazione cellulare e la comprensione dei processi cellulari. Il progetto RNAhello, finanziato dall’UE, sembra aver trovato un modo per studiare la comunicazione basata sull’RNA nelle protocellule artificiali. L’approccio proposto si ispira agli esosomi, vescicole secrete dalle cellule, che trasportano varie macromolecole e agevolano la comunicazione intercellulare. RNAhello creerà queste vescicole partendo dai polimeri e le impiegherà come navette degli mRNA tra popolazioni protocellulari distinte. I risultati forniranno conoscenze fondamentali sul trasferimento degli acidi nucleici e costituiranno uno strumento potente per la scoperta di nuovi farmaci.
Obiettivo
The mimicking of cellular communication pathways and genetic information transfer in synthetic systems is an important target for researchers. These artificial cell systems have the potential to increase understanding of biological and cellular processes, while also having potential applications in healthcare technologies. This project will involve the investigation of exciting new stimuli responsive and highly tuneable hierarchical polymer protocells, and their application in RNA-based communication. Previous generations of polymer protocells have been shown to allow diffusion based communication with small molecules and naked DNA strands – the project will expand upon this by investigating polymersomes as mRNA shuttles between distinct protocell populations. This exosome inspired system will allow the typically unstable mRNA molecules to be protected from degradation in the external medium, thus opening the door to much more lifelike systems, and possible therapeutic applications.
The proposed research project is strongly multidisciplinary, and involves a combination of polymer chemistry, nanomaterials engineering, cell biology, and imaging techniques. RNAhello includes both the transfer of knowledge to the host institution and the training of the candidate in new advanced techniques. Results have the potential to increase understanding of biological mechanisms in relation to the complex hurdle of the nucleic acid based transfer, whilst also providing innovative platform material for treatment of disease. Additional project goals include: engaging with the non-scientific public through outreach methods; gaining of further commercial training for potential exploitation of research; presentation of research results at international conferences; and the preparation of additional funding proposals.
Campo scientifico
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinatore
5612 AE Eindhoven
Paesi Bassi