Description du projet
Produire des lipides microbiens à partir de biomasse lignocellulosique grâce à la biologie synthétique
Les lipides microbiens issus de la bio-industrie constituent une alternative durable possible aux carburants et aux produits chimiques dérivés du pétrole. Le développement commercial des lipides microbiens à partir de matières premières peu coûteuses, comme la biomasse lignocellulosique, est actuellement limité par les coûts de production élevés résultant des prétraitements physico-chimiques et de l’extraction des lipides intracellulaires. Le projet SynBioLipid, financé par l’UE, a ainsi pour objectif de concevoir des organismes modifiés dotés de capacités métaboliques améliorées, en intégrant le prétraitement, la fermentation, la détoxification et la sécrétion de lipides. Le chercheur se servira du champignon Yarrowia lipolytica comme microorganisme modèle pour produire des lipides microbiens à partir de biomasse lignocellulosique, puis développera des stratégies pour surmonter les défis associés à l’expression de grandes voies hétérologues. Il générera des consortiums microbiens synthétiques comprenant des souches spécialisées et utilisera ces communautés pour produire des lipides microbiens à partir de la lignocellulose.
Objectif
Global transition towards a climate-neutral economy demands for the sustainable use of renewable biological resources. Microbial lipids are potential products of bio-based industries and sustainable alternatives to petroleum-derived fuels and chemicals. The commercial development of microbial lipids from inexpensive feedstocks such as lignocellulosic biomass is so far limited mainly due to the elevated production costs imposed by physicochemical pre-treatments and extraction of intracellular lipids. In addition, degradation of lignocellulose releases compounds which are toxic for most of the microorganisms. One solution is to construct engineered organisms with improved metabolic capabilities integrating pre-treatment, fermentation, detoxification and secretion of lipids. Hitherto, there is no successful research on an engineered organism which is able to do all these tasks. In fact, efficient transferring large heterologous pathways into one single microorganism is quite challenging and leads to high metabolic burden and less productivity. SynBioLipid will combine my expertise in metabolic modelling and fermentation using mixed microbial communities with the host experience in synthetic biology. It is aimed at using Yarrowia lipolytica, as a model microorganism for microbial lipids production from lignocellulosic biomass by presenting an innovative and original strategy to overcome the challenges associated with expression of large heterologous pathways. I first will generate synthetic microbial consortia comprised specialist strains, and second, will use these communities for the optimized production of microbial lipids. Each specialist strain is engineered to deliver an optimum output for one or more specific tasks. The metabolic network modelling will be integrated with synthetic biology and metabolic engineering to design and build multifunctional synthetic consortia, enabling efficient lipid production from lignocellulose.
Champ scientifique
- natural sciencesbiological sciencessynthetic biology
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- natural sciencesbiological sciencesmicrobiology
- agricultural sciencesagricultural biotechnologybiomass
- engineering and technologyindustrial biotechnologybioprocessing technologiesfermentation
Mots‑clés
Programme(s)
Régime de financement
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinateur
SW7 2AZ LONDON
Royaume-Uni