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Directed co-evolution via engineered cell-to-cell communication

Descripción del proyecto

Nuevo método de evolución dirigida para profundizar en el control sobre las vías de señalización celular

El proyecto financiado con fondos europeos Evolving together desarrolla un sistema innovador para la coevolución dirigida de los pares celulares ligando-receptor. La plataforma de evolución dirigida célula-a-célula facilitará el desarrollo de vías de señalización intercelulares ortogonales sintéticas para permitir la expresión definida por el usuario de genes diana. La elevada flexibilidad en la selección de los genes indicadores permite personalizar totalmente la respuesta celular posterior, como la programación de patrones celulares autoorganizados o el establecimiento de poblaciones celulares específicas como diana. El proyecto constituye una oportunidad para crear un método de evolución dirigida con impacto en varios campos de la biología y la biomedicina.

Objetivo

The aim of this Marie Skłodowska-Curie Fellowship (Evolving Together) is to develop an innovative new system for directed co-evolution of ligand-receptor pairs in two different cell populations. This novel cell-to-cell directed evolution platform will facilitate the development of synthetic, orthogonal intercellular signaling pathways that can be applied to control and manipulate cells by user-defined expression of a target gene. The selection of the reporter gene can be extremely flexible, which allows full customization of the downstream cellular response, such as programming self-organized cellular patterns or targeting specific cell populations. This Fellowship will be undertaken under the supervision of Prof. Tom Ellis (Imperial College London Centre for Synthetic Biology), who is internationally renowned in synthetic biology and is leading the UK’s effort in the Synthetic Yeast Genome Project (Sc2.0) one of the world’s largest synthetic biology projects to date. My proposed research brings together many aspects of synthetic biology and molecular biology techniques, including molecular cloning, cell culture, protein and genome engineering, directed evolution, and next generation sequencing (NGS). In addition, we will extensively use fluorescence-activated cell sorting (FACS) and confocal imaging to isolate and characterize the populations of interest. These techniques combine skills I’ve acquired during previous research along with the expertise and methods of Tom Ellis’ group. Evolving Together presents an opportunity to make a major new innovation in directed evolution that can impact various fields of biology and biomedicine such as signaling, protein engineering, biomaterials, synthetic biology and therapeutics.

Coordinador

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Aportación neta de la UEn
€ 212 933,76
Dirección
SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
SW7 2AZ LONDON
Reino Unido

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Región
London Inner London — West Westminster
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 212 933,76