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Directed co-evolution via engineered cell-to-cell communication

Description du projet

Nouvelle approche d’évolution dirigée pour un meilleur contrôle des voies de signalisation cellulaire

Le projet Evolving together, financé par l’UE, s’emploie à mettre au point un système innovant pour la coévolution dirigée de paires ligand-récepteur cellulaires. La plateforme d’évolution dirigée de cellule à cellule facilitera le développement de voies de signalisation intercellulaires synthétiques et orthogonales, qui permettront l’expression de gènes cibles définie par l’utilisateur. La grande flexibilité de la sélection des gènes rapporteurs permet une personnalisation complète de la réponse cellulaire en aval, comme la programmation de patrons cellulaires auto-organisés ou le ciblage de populations cellulaires spécifiques. Le projet pourrait permettre de créer une approche d’évolution dirigée ayant un impact sur différents domaines de la biologie et de la biomédecine.

Objectif

The aim of this Marie Skłodowska-Curie Fellowship (Evolving Together) is to develop an innovative new system for directed co-evolution of ligand-receptor pairs in two different cell populations. This novel cell-to-cell directed evolution platform will facilitate the development of synthetic, orthogonal intercellular signaling pathways that can be applied to control and manipulate cells by user-defined expression of a target gene. The selection of the reporter gene can be extremely flexible, which allows full customization of the downstream cellular response, such as programming self-organized cellular patterns or targeting specific cell populations. This Fellowship will be undertaken under the supervision of Prof. Tom Ellis (Imperial College London Centre for Synthetic Biology), who is internationally renowned in synthetic biology and is leading the UK’s effort in the Synthetic Yeast Genome Project (Sc2.0) one of the world’s largest synthetic biology projects to date. My proposed research brings together many aspects of synthetic biology and molecular biology techniques, including molecular cloning, cell culture, protein and genome engineering, directed evolution, and next generation sequencing (NGS). In addition, we will extensively use fluorescence-activated cell sorting (FACS) and confocal imaging to isolate and characterize the populations of interest. These techniques combine skills I’ve acquired during previous research along with the expertise and methods of Tom Ellis’ group. Evolving Together presents an opportunity to make a major new innovation in directed evolution that can impact various fields of biology and biomedicine such as signaling, protein engineering, biomaterials, synthetic biology and therapeutics.

Coordinateur

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Contribution nette de l'UE
€ 212 933,76
Adresse
SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
SW7 2AZ LONDON
Royaume-Uni

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Région
London Inner London — West Westminster
Type d’activité
Higher or Secondary Education Establishments
Liens
Coût total
€ 212 933,76