CORDIS - Forschungsergebnisse der EU
CORDIS

Directed co-evolution via engineered cell-to-cell communication

Projektbeschreibung

Neuer Ansatz der gerichteten Evolution zur Steuerung von Zellsignalwegen

Das EU-finanzierte Projekt Evolving together entwickelt ein innovatives System für die gerichtete Koevolution zellulärer Ligand-Rezeptor-Paare. Die Plattform für die gerichtete Zelle-zu-Zelle-Evolution wird die Entwicklung synthetischer, orthogonaler interzellulärer Signalwege vereinfachen und so die benutzerdefinierte Expression von Zielgenen ermöglichen. Die hohe Flexibilität bei der Auswahl der Reportergene erlaubt die vollständige Anpassung der abwärtsgerichteten Zellreaktion, wie das Programmieren selbstorganisierender Zellmuster oder die Ausrichtung auf spezifische Zellpopulationen. Das Projekt bietet die Möglichkeit, einen Ansatz der gerichteten Evolution mit Relevanz für verschiedene Bereiche der Biologie und Biomedizin zu schaffen.

Ziel

The aim of this Marie Skłodowska-Curie Fellowship (Evolving Together) is to develop an innovative new system for directed co-evolution of ligand-receptor pairs in two different cell populations. This novel cell-to-cell directed evolution platform will facilitate the development of synthetic, orthogonal intercellular signaling pathways that can be applied to control and manipulate cells by user-defined expression of a target gene. The selection of the reporter gene can be extremely flexible, which allows full customization of the downstream cellular response, such as programming self-organized cellular patterns or targeting specific cell populations. This Fellowship will be undertaken under the supervision of Prof. Tom Ellis (Imperial College London Centre for Synthetic Biology), who is internationally renowned in synthetic biology and is leading the UK’s effort in the Synthetic Yeast Genome Project (Sc2.0) one of the world’s largest synthetic biology projects to date. My proposed research brings together many aspects of synthetic biology and molecular biology techniques, including molecular cloning, cell culture, protein and genome engineering, directed evolution, and next generation sequencing (NGS). In addition, we will extensively use fluorescence-activated cell sorting (FACS) and confocal imaging to isolate and characterize the populations of interest. These techniques combine skills I’ve acquired during previous research along with the expertise and methods of Tom Ellis’ group. Evolving Together presents an opportunity to make a major new innovation in directed evolution that can impact various fields of biology and biomedicine such as signaling, protein engineering, biomaterials, synthetic biology and therapeutics.

Koordinator

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Netto-EU-Beitrag
€ 212 933,76
Adresse
SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
SW7 2AZ LONDON
Vereinigtes Königreich

Auf der Karte ansehen

Region
London Inner London — West Westminster
Aktivitätstyp
Higher or Secondary Education Establishments
Links
Gesamtkosten
€ 212 933,76