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Comparative modelling of organoids in the age of single-cell transcriptomics

Project description

Computational framework for organoid comparative modelling

The goal of the EU-funded OrganoidAlign project is to develop an organoid comparative modelling computational framework for tissue engineering and single-cell genomics studies. The objective is to build a set of probabilistic models for aligning single-cell transcriptomic profiles of organoids versus their in vivo tissue. The study will use a new statistical framework for comprehensive comparisons between in vitro and in vivo pairs of transcriptomic profiles. The framework application will help in the prediction of missing or outlying cellular components, transcriptional factors and signalling pathways in organoids compared to their in vivo tissues, providing directions for organoid protocol improvement.

Objective

The OrganoidAlign action will develop a solid computational framework for comparative modelling of organoids in the age of single-cell transcriptomics. It will provide direct payoffs to both, tissue engineering and single-cell genomics fields. The overarching goal is to build a set of statistically rigorous and consistent probabilistic models for aligning a single-cell transcriptomic profile of an organoid against its in vivo tissue, for quantitatively evaluating its recapitulatory power.

During the course of this action, a meticulous review will be conducted on the state-of-the-art single-cell data modelling and comparative analysis techniques prior to formulating the single-cell transcriptomic profile comparison problem in statistical learning theory. This will specifically focus on both cell clustering and cell trajectory inference methods. A new statistical framework will be implemented to accommodate a comprehensive comparison between a pair of in vitro and in vivo transcriptomic profiles. A rigorous scoring measure will be devised to quantify their alignment.

Overall, the inference components under the proposed framework will facilitate the prediction of missing or outlying cellular attributes, transcriptional factors and signaling pathways in organoids compared to their in vivo tissues, informing directions of organoid protocol improvement. Overall, its outcomes will have potential contributions towards engineering more reliable in vitro tissue models, as well as reference profiling of organoids under the Human Cell Atlas project.

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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(opens in new window) H2020-MSCA-IF-2020

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Coordinator

GENOME RESEARCH LIMITED LBG
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 212 933,76
Address
WELLCOME SANGER INSTITUTE WELLCOME GENOME CAMPUS HINXTON
CB10 1SA SAFFRON WALDEN
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 212 933,76
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