Projektbeschreibung
Präziser Schnelltest auf Infektionserreger
Das Verfahren der Polymerase-Kettenreaktion (PCR) war ein Durchbruch in der Molekularbiologie mit vielfältigsten Anwendungsmöglichkeiten, etwa dem Nachweis von Bakterien- und Vireninfektionen. Ein Nachteil der ansonsten hochsensitiven Methode ist allerdings noch immer der hohe Zeit- und Arbeitsaufwand. So entwickelt das EU-finanzierte Projekt NORAMID SE für einen PoC-Schnelltest (patientennahe Labordiagnostik) einen Mikrochip, der Nukleinsäure-DNA aus biologischen Proben isoliert, in ein Mikrofluidiksystem integriert ist und so die hochpräzise Diagnose von Infektionserregern ermöglicht. Da das Ergebnis bereits nach 20 Minuten vorliegt, vereinfacht NORAMID SE die Schnelldiagnose von Krankheitserregern.
Ziel
The catastrophic impact of infectious diseases including the recent coronavirus (covid-19) pandemic on global health cannot be over emphasized. Polymerase chain reaction (PCR) based techniques are the most sensitive and reliable diagnostic tools for infectious diseases but are time-consuming and labour-intensive. This project aims to develop a simple, rapid and cost-effective novel nucleic acid (NA) isolation microchip and integrate it in the FASTGENE PCR microfluidics device for point of care (POC) diagnosis of infectious diseases. The project will exploit the competence of the experience researcher (ER) on the theoretical and practical knowledge of medical biotechnology and molecular epidemiology while implementing the expertise of BforCure team in microfluidics design to develop this new microchip. The ER will develop a new NA isolation microchip based on silica gel solid phase extraction system and integrate it in the FASTGENE PCR microfluidics device of BforCure to become a closed system device that automatically extracts NA and amplifies it to detect infectious diseases. After the design and production, the device will be tested for its efficiency. Once satisfied, positive control bacteria and viral samples will be used to assess the performance of the device and the results will be compared with that of standard commercially available NA extraction and PCR kits. The device is expected to have high sensitive compared to other microfluidics devices, reliable and can detect pathogens in less than 20 minutes. A patent will be secured for the product while the findings of the research will be made public and commercialized. This novel device will be suitable for POC diagnosis to promptly detect and manage infectious diseases. This project will develop the ER’s potential to transform research ideas to products and build up his industrial experience. It will make the ER gain expertise in the design and fabrication of microfluidics tools and develop his entrepreneuri
Wissenschaftliches Gebiet
- natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidics
- medical and health sciencesmedical biotechnology
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- medical and health scienceshealth sciencespublic healthepidemiologypandemics
- medical and health scienceshealth sciencesinfectious diseasesRNA virusescoronaviruses
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenFinanzierungsplan
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
93100 Montreuil
Frankreich
Die Organisation definierte sich zum Zeitpunkt der Unterzeichnung der Finanzhilfevereinbarung selbst als KMU (Kleine und mittlere Unternehmen).