Descrizione del progetto
Sviluppare una materia prima composta da un solo atomo di carbonio
Il metanolo a un solo atomo di carbonio (C1) è una promettente materia prima alternativa per ridurre la dipendenza da combustibili fossili. Abbondante ed economico, si potrebbe produrre in maniera sostenibile dai rifiuti, dalla CO2 e dall’idrogeno rinnovabile. Il progetto ADVANC1-METH, finanziato dall’UE, progetterà un percorso ad alta efficienza di assimilazione del metanolo sintetico nel noto batterio ospite industriale Escherichia coli. Per i passaggi più complessi sarà eseguita un’ottimizzazione modulare in modo da raggiungere un’efficiente conversione enzimatica del metanolo nella cella. Le prestazioni complessive del ceppo saranno ottimizzate attraverso un targeting razionale e sistematico della rete metabolica e i ceppi a crescita rapida sul metanolo saranno selezionati e studiati utilizzando un innovativo e altamente produttivo approccio di editing del genoma.
Obiettivo
Urgent global problems, including climate change and fossil-fuel dependence, force the EU to develop sustainable bio-basedchemical production platforms. However, current biotechnological feedstocks, such as plant-based sugars, suffer from serious limitations in terms of availability and sustainability. A very promising alternativeis the one-carbon (C1) feedstock methanol, which canbe produced in a sustainable manner from waste, or CO2 and renewable hydrogen. So far, the conversion of methanol to products is limited by the lack of suitable, efficientmicrobial cell factories. Hence, this project will engineer a highly efficient, synthetic methanol assimilation pathway into the well-known industrial host bacterium Escherichia coli. First, modular optimization will be performed for the bottleneck steps of enzymatic methanol conversion in the cell. Then, the overall strain performance will be optimized by rational, systems-wide targeting of the metabolic network and fast growing strains on methanol will be selected and studied. To achieve the objectives an innovative high-throughput genome editing approach will be employed. ADVANC1-METH is expected to yield a bacterial platform strain for efficient conversion of methanol to products, and industrially relevant IP and scientific knowledge will be effectively disseminated. The ADVANC1-METH project will aid the applicant to further extend his knowledge in the interdisciplinary fields of synthetic biology, bioinformatics, genetic and metabolic engineering, and complement his academic skills to fulfil the ambition to become an independent researcher. The implementation and training will be hosted at an institute with key expertise in bacterial and metabolic engineering. Within the ADVANC1-METH project videos and a board game will be developed to increase public engagement and knowledge on sustainable and methanol-based biotechnology, which are essential to realize a sustainable bio-based society.
Campo scientifico
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinatore
6708 PB Wageningen
Paesi Bassi