Descripción del proyecto
Un nuevo enfoque biocompatible para lograr la endosimbiosis artificial
Los endosimbiontes son microorganismos que viven en el interior de un organismo huésped, a menudo en una relación mutualista. Dos grandes tipos de orgánulos de las células eucariotas, las mitocondrias y los plastos (como los cloroplastos), se consideran endosimbiontes bacterianos. Inspirada en la naturaleza, la biología sintética ha permitido ajustar la biología molecular del huésped para adaptarla a otro organismo con el objetivo final de desarrollar vías metabólicas y circuitos genéticos completos. El enmascaramiento de los huéspedes con polímeros biocompatibles aumentará nuestra capacidad de lograr la endosimbiosis artificial. Financiado por las Acciones Marie Skłodowska-Curie, el equipo del proyecto PolyMAE investigará una novedosa técnica para encapsular procariotas en carcasas biocompatibles, ofreciendo así una nueva vía para la producción sencilla de endosimbiontes artificiales bioortogonales.
Objetivo
Synthetic biology has constantly been expanding its reach, aiming to develop whole genetic circuits and metabolic pathways, which require laborious genetic engineering. Often, such pathways derive from other organisms and must be tweaked to fit the host’s molecular biology, adding to the complexity of the task. However, nature has already witnessed the integration of whole prokaryotic organisms into eukaryotic hosts, where they are integrated as endosymbionts creating new combinations that live with mutual benefit. In evolutionary history, this phenomenon was at the origin of organelles such as mitochondria and chloroplasts, which added their own metabolism to their hosts. To achieve a more generalized framework, where any prokaryote can enter endosymbiosis with any eukaryote, we need to induce the uptake and retention of the guest by the eukaryotic host. A way forward will be by masking the guests with biocompatible polymers, either pre-formed, or synthesized by the bacterium itself. The project proposed herein offers a novel technique for the the encapsulation of prokaryotes into biocompatible shells, creating a new strategy for the straightforward production of bio-orthogonal, artificial endosymbionts. After the formation of the polymer shell around bacteria, they will be endocytoted by eukaryotes, thus expanding the biochemical potentialities of cells, revolutionizing the possibilities of synthetic biology, acting as true artificial organelles. This breakthrough will allow the functional insertion whole genomes into the host cells and the combination of micro-organisms, which will have implications in medicinal, industrial and environmental biotechnology. It will lead to the creation of complex, semi-synthetic hybrid organisms able to perform a vast variety of non-natural biotransformations. In line with the 2020 Work Programme, it will open up several diverse possibilities that will benefit European and global industries.
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
64289 Darmstadt
Alemania