Descrizione del progetto
Un nuovo approccio biocompatibile per realizzare l’endosimbiosi artificiale
Gli endosimbionti sono organismi che vivono all’interno di un organismo ospite, spesso in una relazione mutualistica. Due tipi principali di organelli nelle cellule eucariotiche, i mitocondri e i plastidi come i cloroplasti, sono considerati endosimbionti batterici. Ispirandosi alla natura, la biologia sintetica ci ha permesso di modificare la biologia molecolare dell’ospite per adattarla a un altro organismo, con lo scopo ultimo di sviluppare interi circuiti genetici e percorsi metabolici. Mascherando gli ospiti con polimeri biocompatibili, aumenterà la nostra capacità di realizzare l’endosimbiosi artificiale. Finanziato dal programma di azioni Marie Skłodowska-Curie, il progetto PolyMAE studierà una nuova tecnica per incapsulare i procarioti in gusci biocompatibili, offrendo una nuova via per la produzione diretta di endosimbionti artificiali bio-ortogonali.
Obiettivo
Synthetic biology has constantly been expanding its reach, aiming to develop whole genetic circuits and metabolic pathways, which require laborious genetic engineering. Often, such pathways derive from other organisms and must be tweaked to fit the host’s molecular biology, adding to the complexity of the task. However, nature has already witnessed the integration of whole prokaryotic organisms into eukaryotic hosts, where they are integrated as endosymbionts creating new combinations that live with mutual benefit. In evolutionary history, this phenomenon was at the origin of organelles such as mitochondria and chloroplasts, which added their own metabolism to their hosts. To achieve a more generalized framework, where any prokaryote can enter endosymbiosis with any eukaryote, we need to induce the uptake and retention of the guest by the eukaryotic host. A way forward will be by masking the guests with biocompatible polymers, either pre-formed, or synthesized by the bacterium itself. The project proposed herein offers a novel technique for the the encapsulation of prokaryotes into biocompatible shells, creating a new strategy for the straightforward production of bio-orthogonal, artificial endosymbionts. After the formation of the polymer shell around bacteria, they will be endocytoted by eukaryotes, thus expanding the biochemical potentialities of cells, revolutionizing the possibilities of synthetic biology, acting as true artificial organelles. This breakthrough will allow the functional insertion whole genomes into the host cells and the combination of micro-organisms, which will have implications in medicinal, industrial and environmental biotechnology. It will lead to the creation of complex, semi-synthetic hybrid organisms able to perform a vast variety of non-natural biotransformations. In line with the 2020 Work Programme, it will open up several diverse possibilities that will benefit European and global industries.
Campo scientifico
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinatore
64289 Darmstadt
Germania