Addressing the impact of surface ectoderm and somitic mesoderm development on neural tube morphogenesis.

Informazioni relative al progetto

Neural Tube

ID dell’accordo di sovvenzione: 101038073

DOI

10.3030/101038073

Progetto concluso il 1 Gennaio 2023

Data della firma CE 2 Aprile 2021

Data di avvio 1 Ottobre 2021

Data di completamento 30 Settembre 2023

Finanziato da

• SPREADING EXCELLENCE AND WIDENING PARTICIPATION

Costo totale

€ 157 941,12

Contributo UE

€ 157 941,12

Coordinato da

UNIVERSITY OF CYPRUS
Cyprus

Periodic Reporting for period 1 - Neural Tube (Addressing the impact of surface ectoderm and somitic mesoderm development on neural tube morphogenesis.)

Periodo di rendicontazione: 2021-10-01 al 2023-09-30

Sintesi del contesto e degli obiettivi generali del progetto

One of the most important events during vertebrate embryogenesis is the formation of the central nervous system. The first critical event during central nervous system development is the formation of the neural tube. The neural tube emerges from the dorsal ectoderm and is the precursor of the brain and spinal cord. Defective neural tube formation leads to neural tube defects which are one of the most common human birth defects, occurring in 0.5-2 per 1000 births. Thus
understanding the morphogenetic events driving neural tube formation will give us a better understanding of human neural tube defects and facilitate their prevention. This project focused on the role of the surface ectoderm and somitic mesoderm during neural tube closure. T

The research objectives of this projects were:
1)Examine the impact of surface ectoderm development on neural tube closure
2) Examine the impact of somitic mesoderm convergent extension on neural tube closure.

Additionally the project had specific training objectives for fellow's career evelopment.

The training objectives were:

1) Development of new research skills by the experienced researcher.
2)Development of skills in communication and outreach.
3) Enhancement of mentoring skills.
4)Building solid skills in writing
5)Develop skills in grant writing and funding acquisition.

Lavoro eseguito dall’inizio del progetto fino alla fine del periodo coperto dalla relazione e principali risultati finora ottenuti

The data generated during the implementation of this project revealed that the behaviour of tissues mechanically coupled with the neuroepithelium is necessary for proper central nervous system morphogenesis. Specifically here we showed that both surface ectoderm and somitic mesoderm development are essential for proper neural tube formation. Defective neural tube formation leads to neural tube defects, which are one of the most common human birth defects. Thus our work indicated that t neural tube defects can be categorized in 1) neural tube defects stemming from defects in neural plate intrinsic morphogenetic processs and 2) neural tube defects emerging from defective morphogenesis of tissues mechanically coupled with the neuroepithelium.


Dissemination:
The data generated in this project led to two peer reviewed open access publications. Additionally this work was presented in a conference and in departmental seminar.

Additionally the fellow through social media post communicated the project's finding to the scientific community.

Furthermore this work was communicated to the general public through press release to Cypriot media.

Last the fellow participated to 2022 European Researcher's night were he has the chance to communicate the importance of his reearch and MSCA actions to university and school student.

Exploitation:

Data generated in this project were used by the fellow to submit a proposal for the call Excellence from Cyprus Research and Innovation foundation. This proposal was selected for funding (200000 euro).

Additionally the fellow submitted an ERC-StG proposal in the course of this action. This proposal was not selected for funding.

Progressi oltre lo stato dell’arte e potenziale impatto previsto (incluso l’impatto socioeconomico e le implicazioni sociali più ampie del progetto fino ad ora)

The appearance of neural tube defects in human births has been significantly reduced due to folic acid supplementation. However, is it well known that at least one third of neural tube defects are not responsive to folate. The data generated during the implementation of this project revealed that neural tube defects can be elicited by neural plate constraint defects or by defects in the mechanically coupled tissues surface ectoderm and somitic mesoderm. our work suggests that distinct categories of neural tube defects will respond differently to folate supplementation. Therefore, future studies should focus on the responsiveness of it will neural tube defects emerging from defects in mechanically coupled tissues ato folate supplementation.

Dorsal view of neurula stage Xenopus embryo