Description du projet
Des bactéries amatrices d’extrêmes pourraient stimuler la production de solvants
Produisant de nombreux produits chimiques précieux grâce à la biologie synthétique et à l’ingénierie métabolique, les microbes apparaissent comme des alliés prometteurs dans notre transition vers une énergie plus verte. Toutefois, l’augmentation de la production se heurte à la toxicité des substances chimiques produites pour les microbes de production ordinaires. Ce problème n’en est peut-être pas un pour Pseudomonas, qui s’avère être un extrémophile, un organisme pouvant survivre dans des environnements extrêmes. Le projet PROSPER, financé par l’UE, permettra de modifier cet organisme robuste de manière à ce qu’il fournisse un puits pratiquement inépuisable de solvants hydrophobes tels que le styrène et le benzène à l'aide d’une nouvelle approche n’ayant jamais fait l’objet d’une démonstration.
Objectif
Replacement of fossil chemicals with biological counterparts has been widely accepted as a vital pursuit to increase the sustainability of our chemical and material industries. Synthetic biology and metabolic engineering enable us to produce a plethora of chemicals with microbes, but the majority of these never make it past the proof-of-principle stage. This is especially the case for drop-in bulk aromatics like styrene or benzene. The main reason for this is that such products are too toxic to ordinary production microbes.
In PROSPER I aim to overcome this hurdle and demonstrate the efficient microbial production of hydrophobic aromatic chemicals using solvent-tolerant Pseudomonas. I will engineer this unique extremophile to break the solubility barrier of these chemicals, forming a second phase of product. This second phase provides a virtually endless product sink and it enables extremely simple downstream recovery.
The bio-based production of a second phase of such chemicals has thus far never been shown. I believe that this relates to a fundamental problem in biotechnology: production tolerance, i.e. tolerance of the producing organism to the produced product, rather than to an externally added chemical (as it is usually studied). In PROSPER I intend to generate deep mechanistic insights into the processes governing both types of tolerance and to leverage these insights to open up a new field of biotechnological production of hydrophobic compounds. To achieve this, I will develop new methods to analyze intracellular solvent concentrations, build a Pseudomonas chassis with enhanced production tolerance to hydrophobic solvents, and enable production of solvents like styrene, ethylbenzene, and even benzene.
I am in a unique position to achieve this goal, with over 15 years of experience in the engineering of Pseudomonas as a workhorse in biotechnology, the study of solvent-tolerance, and the development and application of synthetic biology tools and metho
Champ scientifique
Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régime de financement
HORIZON-AG - HORIZON Action Grant Budget-BasedInstitution d’accueil
52428 Julich
Allemagne