Descrizione del progetto
Microscopia tomografica per l’analisi istopatologica intraoperatoria
L’istologia, la valutazione dei tessuti e delle cellule al microscopio, è fondamentale per la diagnosi, la stadiazione e la gestione del trattamento del cancro. Richiede il prelievo di biopsie, che vengono sezionate e colorate per essere analizzate successivamente. Tuttavia, il protocollo di colorazione non è applicabile in vivo e l’analisi finale del campione di tessuto è possibile solo dopo diverse ore. Il progetto SpeckleCARS, finanziato dall’UE, si propone di sviluppare una microscopia tomografica veloce e senza marcature, con sensibilità vibrazionale, per ottenere immagini 3D con una qualità paragonabile ai campioni colorati tradizionali. L’uso di uno schema di riflessione ad ampio campo e il contrasto senza marcature non richiedono la rimozione dei tessuti e forniscono dati istologici 3D intraoperatori istantanei.
Obiettivo
Histology is central to the diagnosis, staging, and treatment of cancers. It requires the removal of small regions of suspect tissues (biopsies) that are later sectioned and stained with haematoxylin and eosin (HE). However, histology suffers from major weaknesses: First the standard HE protocol cannot work in vivo and requires ex vivo biopsies. Second HE is labour intensive, time consuming and the final tissue sections inspection is possible only after 12 hours. A faster but less accurate protocol, known as extemporaneous histology, can be performed in 40 min and is used in an intra-operative context to guide surgeries. The SpeckleCARS project aims to develop for the first time fast label free tomography microscopy with vibrational sensitivity to provide images with tridimensional improvement as compared to extemporaneous histology and in near-perfect concordance with conventional stained HE histology. This pioneering approach overcomes all the previous challenges: Using a wide field reflection scheme and label free contrast rules out all the drawbacks due to tissue removal and external labelling. It provides instantaneous intraoperative 3D histological data, improving the accessibility and accuracy of tumour detection, diagnosis and treatment. Combining the latest advances in 3D tomography reconstruction algorithms, high power laser systems together with key innovations in random speckle illumination and hyperspectral vibrational imaging, the SpeckleCARS project will provide for the first time volumetric histology imaging in real time over large (mm) fields of view without the need of tissue removal. To demonstrate the power of the developed technologies both ex vivo human and in vivo mouse cancer assessments will be conducted in collaboration with pathologists. The SpeckleCARS approach is broadly applicable to all cancer types and to any tissue diagnostic requiring histological data, so the project breakthroughs will benefit many medical and biology applications.
Campo scientifico
Programma(i)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Argomento(i)
Meccanismo di finanziamento
ERC - Support for frontier research (ERC)Istituzione ospitante
75794 Paris
Francia