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Circular RNA Therapeutics for Duchenne Muscular Dystrophy

Descripción del proyecto

Un método innovador con ARN circular para curar la distrofia muscular

Los tratamientos con ARN son una nueva arma contra diversas enfermedades, como los trastornos genéticos, el cáncer, las inflamaciones y las infecciones víricas. Sin embargo, la vida relativamente corta del ARN constituye un obstáculo para su aplicación en tratamientos sustitutivos con proteínas. El ARN circular (ARNcirc) es un ARN monocatenario de circuito cerrado más estable que el ARN lineal. Además, las nanopartículas lipídicas (NPL) son las mejores candidatas para la administración de ARN, ya que pueden contener grandes cargas de ARN. La distrofia muscular de Duchenne (DMD) está asociada a una mutación recesiva y disfuncional de la proteína distrofina. El equipo del proyecto circRNA4DMD, financiado con fondos europeos, propone un método innovador para administrar NPL cargadas con ARNcirc a los miocitos. El objetivo es promover la expresión a largo plazo de la distrofina, reemplazando así la proteína defectuosa en un modelo murino de DMD.

Objetivo

RNA therapeutics is an emerging field explored in various types of diseases such as genetic disorders, cancer, inflammation and viral infections. Currently, most of the research focuses on the delivery of mRNA molecules that will transiently express a desired protein that can replace a defective protein or manipulate gene expression in the cells. My lab was the first to show systemic, cell-specific delivery of mRNA molecules in animals. Our approach and our novel amino lipids were translated to several clinical trials in the field of infectious and monogenic diseases.
In protein replacement therapy, the main hurdle of using mRNA is the relative short half life of the mRNA. To address this, I suggest an approach for long-term expression: Circular RNA (circRNA), a covalently closed loop single stranded RNA that has a significant prolonged stability compared to linear mRNA. Thus, presents an immense advantage in protein replacement therapy.
Duchenne muscular dystrophy (DMD) is caused by X-linked recessive mutation in dystrophin gene, leading to lack of functional dystrophin protein. This disease affects 1:5,000 males, causes a progressive loss of muscle tissues, ultimately leading to disability and premature death. Because DMD pathology is caused by the lack of functional dystrophin, restoring the function of dystrophin is a potential therapeutic strategy.
As Lipid nanoparticles (LNPs) are the most clinically advanced candidate for RNA delivery, able to entrap large RNA payloads, herein I propose an innovative multidisciplinary approach for the specific delivery of circRNA-LNPs to muscle cells that will express the dystrophin protein and replace the defective one in a DMD mouse model.
The long-term expression of the circRNA will offer new hope for the treatment of monogenetic diseases such as DMD. This approach may ultimately become a novel therapeutic modality for DMD and open new avenues for implementing circRNAs for other types of genetic disorders and vaccines

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural.

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Régimen de financiación

HORIZON-ERC - HORIZON ERC Grants

Institución de acogida

TEL AVIV UNIVERSITY
Aportación neta de la UEn
€ 2 500 000,00
Coste total
€ 2 500 000,00

Beneficiarios (1)