The application of ecological principles and concepts in farming and food systems gave rise to a holistic approach called agroecology, which provides new agricultural management approaches. Agroecology-TRANSECT aims to realize the full potential of agroecology both as a concept, and a set of practices and policy proposals, and quantify the effects of agoecology on climate change adaptation and mitigation, biodiversity preservation in agro-ecosystems, and farm socio-economic resilience. A challenge is however that the term agroecology is interpreted differently in scientific, social and political arenas, which can lead to false equivalencies and confusion. We therefore reviewed i) scientific evidence on the effects of agroecological interventions on biodiversity and climate change mitigation; ii) the narratives that frame agroecological social movements in Europe; and iii) how key instruments of the Common Agricultural Policy (CAP) influence agroecological farming practices. Taking a place-based approach, we aim to empirically learn from and support the transition to agroecology in 11 multi-actor Innovation Hubs (IHs), focused on annual and perennial cropping, grassland-based and integrated crop-livestock systems across Europe, through a co-innovation approach to project governance. From this dialogue, we are continually identifying drivers, barriers, policies, market powers and social norms that generate opportunities to enhance the adoption of agroecology. We are acting to ensure that the knowledge generated in the project serves decision-making from regional to EU level.