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Development of a multilayered and bioprinted iPSC-derived retinal model with use in preclinical and toxicological testing

Project description

Innovative bioprinted retinal model for macular degeneration drug discovery

Age-related macular degeneration (AMD) is the most prevalent cause of vision loss in the elderly. Current 2D-cellular, organoid-based, and animal models are unable to faithfully recapitulate the pathophysiology of the disease, complicating the development of efficient treatments. Funded by the Marie Skłodowska-Curie Actions programme, the iRETINA project aims to develop bioprinting of an in vitro human retinal model using endothelial and retinal cells derived from induced pluripotent stem cells (iPSCs). The goal is to create a functional, vascularised iPSC-derived tissue model of the native stratified retina for preclinical drug development, toxicological evaluation, and the study of basic mechanisms of complex immune response changes leading to AMD.

Objective

Age-related macular degeneration (AMD) encompass the most prevalent cause of vision-loss in elderly people in developed countries. One of the main hurdles restraining the development of efficient treatments against AMD is the inability of current animal, 2D-cellular and organoid-based models to completely recapitulate the pathophysiology of this disease. The goal of the iRETINA project is to bioprint an in-vitro human retinal model using isolated endothelial and retinal cells derived from iPSCs. The result will be a functional and vascularized iPSC-derived tissue resembling the native stratified retina that could serve as a preclinical and toxicological model for drug development and for the study of complex phenomena such as allogenic immune response. In order to achieve this goal, the iRETINA project will focus at three different levels: 1) implementing state-of-the-art genome editing tools for the development of retinal cell differentiation and enrichment strategies from healthy and AMD-patient iPSC; 2) developing new methodologies for bioprinting endothelial and retinal cells aiming to preserve cell viability, synaptic connectivity and structural integrity; 3) proof of concept testing to evaluate the usefulness of the artificial retina as a bona fide in vitro retinal model. The nature of this project is strongly multidisciplinary and involves the collaboration of basic researchers with experts in the fields of ophthalmology, immunology and biomaterial sciences. The results of this proposal have the potential to represent a true technological and scientific breakthrough that will result in better organ and disease modeling, and reduced laboratory animal use, eventually minimizing the burden and complexity of preclinical trials required for the development of novel pharmacological and cell replacement therapies against AMD.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2021-PF-01

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Coordinator

FUNDACION PUBLICA ANDALUZA PROGRESO Y SALUD M.P.
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 206 641,20
Address
AVENIDA AMERICO VESPUCIO 15 EDIF S2
41092 Sevilla
Spain

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Region
Sur Andalucía Sevilla
Activity type
Research Organisations
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Total cost

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