Description du projet
Conceptions pour des souches microbiennes plus robustes
La robustesse microbienne est importante pour une production durable et efficace basée sur la biotechnologie. Les souches de producteurs créées en laboratoire ne répondent pas toujours bien aux conditions industrielles telles que les grands volumes de fermentation, l’accumulation d’intermédiaires toxiques et l’acidification du milieu. Le projet BioCircus, soutenu par la programme Actions Marie Skłodowska-Curie (MSCA), s’attaquera au problème du manque de robustesse des souches microbiennes modifiées. Plus précisément, il s’agira d’explorer la construction de biocapteurs et de circuits de régulation dynamique qui mesurent et contrôlent la production de la molécule cible. Il utilisera également des modèles prédictifs d’apprentissage automatique et l’automatisation pour améliorer ces circuits. La naringénine, une molécule clé de la famille des flavonoïdes, sera utilisée comme molécule de preuve de principe. E. coli sera utilisé comme organisme modèle.
Objectif
Biological production of chemicals and materials is a renewable and economically viable alternative to the current chemical processes, easing the transition towards a biology-based circular economy. The current biomanufacturing industry faces a scalability problem. Frequently, producer strains created in the laboratory do not respond well to industrial conditions (e.g. large fermentation volumes, accumulation of toxic intermediates, media acidification, etc.). The goal of BioCircus is to address the issue caused by the lack of robustness of the engineered microbial strains. BioCircus is based on three main research pillars: (1) the construction of biosensor and dynamic regulation circuits that measure and control the production of the target molecule, (2) the use of machine learning predictive models and automation to improve these circuits and (3) validating the designed strains in pre-industrial conditions, closer than standard molecular biology laboratory procedures to real-world bioproduction operations. Naringenin, a key molecule of the flavonoid family, will be used in as proof-of-principle molecule. E. coli will be used as model organism. The design and construction of biosensors and dynamic regulation circuits controlling naringenin production is expected to support the development of methodologies and tools suitable for any other detectable metabolite. BioCircus will produce academic and industrial impacts that will boost the bioproduction field by enabling a shorter strain time-to-market for a wide variety of adaptive and scalable microbial bioproducer strains.
Champ scientifique
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensors
- social scienceseconomics and businesseconomicssustainable economy
- natural sciencescomputer and information sciencesartificial intelligencemachine learning
- engineering and technologyindustrial biotechnologybioprocessing technologiesfermentation
- natural sciencesbiological sciencesmolecular biology
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-AG-UN - HORIZON Unit GrantCoordinateur
46022 Valencia
Espagne