Description du projet
Microbiome synthétique contre les maladies du tronc de la vigne
Ces deux dernières décennies, les maladies du tronc de la vigne (MTV) touchent de plus en plus la vigne (Vitis vinifera L.). Ces maladies constituent sans conteste le plus grand défi pour la viticulture moderne. En effet, cela fait plus d’un siècle que les viticulteurs ont recours à des fongicides, des insecticides, des herbicides et des engrais de synthèse. Selon des recherches récentes, ces traitements sont susceptibles d’avoir engendré un déséquilibre dans le microbiome endophyte de la vigne. Ce constat fournit une explication possible du succès récent des agents pathogènes associés aux MTV. Le projet WildWoodMicrobes, financé par l’UE, associera pathologie végétale, épigénétique et écologie microbienne et étudiera les populations sauvages de V. vinifera subsp. sylvestris (VVS). Le projet mettra au jour la diversité du microbiome endophyte des populations sauvages de VVS, réintroduira des endophytes isolés de VVS dans des vignes cultivées et évaluera l’efficacité du microbiome synthétique dans la lutte contre les pathogènes associés aux MTV.
Objectif
In recent years, a general decline has been documented in several agriculturally relevant woody plants, and numerous
reports point towards a possible role of the plant-associated microbiome. This especially applies to grapevines (Vitis vinifera
L.) and to the recent outbreak of grapevine trunk diseases (GTD). This cluster of fungal diseases is considered the greatest
challenge in modern viticulture, due to the complexity of the different pathosystems and the lack of reliable control strategies.
During the last >100 years, vineyards have been routinely treated with fungicides, insecticides, herbicides and fertilizers.
Recent research have demonstrated that such treatments and other anthropic intervention (e.g. domestication, grafting,
training) affect the plant-associated microbiome. These human-driven alterations, exacerbated by grapevines clonal
propagation process, are believed to have led to an imbalance in grapevines endophytic microbiome, which may be a key
explanation for the recent success of GTD-associated pathogens. The best candidates to investigate the composition of a
balanced wood microbiome, unaffected by anthropic activities, are wild populations of V. vinifera subsp. sylvestris (VVS).
In this multidisciplinary project, I will join the fields of plant pathology, epigenetics and microbial ecology to (1) unravel the endophytic
microbiome diversity of wild populations of VVS, using DNA metabarcoding and in vitro isolation techniques; (2) re-introduce
endophytes isolated from VVS in cultivated grapevines, by means of a synthetic microbiome transfer; (3) assess the efficacy
of the synthetic microbiome in antagonizing GTD-associated pathogens, a next-generation approach to biological control.
The outcomes of this project will not only contribute to advance our understanding of grapevine endophytes ecology, but
have also the potential to provide a concrete solution to viticulture, for the benefit numerous European and non-European
countries.
Champ scientifique
Mots‑clés
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinateur
33100 Udine
Italie