Descrizione del progetto
Microbioma sintetico contro le patologie del tronco della vite
Negli ultimi due decenni, le cosiddette malattie del legno della vite (GTD) hanno colpito sempre di più le viti (Vitis vinifera L.). Indubbiamente, queste patologie rappresentano la più grande sfida per la viticoltura moderna. I viticoltori impiegano fungicidi, insetticidi, erbicidi e fertilizzanti sintetici da oltre un secolo. Secondo studi recenti, tali trattamenti potrebbero aver causato uno squilibrio nel microbioma endofitico della vite. Ciò fornisce una possibile spiegazione alla recente diffusione di patogeni associati a GTD. Il progetto WildWoodMicrobes, finanziato dall’UE, combinerà fitopatologia, epigenetica ed ecologia microbica e studierà le popolazioni selvatiche di V. vinifera subsp. sylvestris (VVS). Il progetto svelerà la diversità del microbioma endofitico delle popolazioni selvatiche di VVS, reintrodurrà endofiti isolati da VVS in viti coltivate e valuterà l’efficacia del microbioma sintetico nel contrastare i patogeni associati a GTD.
Obiettivo
In recent years, a general decline has been documented in several agriculturally relevant woody plants, and numerous
reports point towards a possible role of the plant-associated microbiome. This especially applies to grapevines (Vitis vinifera
L.) and to the recent outbreak of grapevine trunk diseases (GTD). This cluster of fungal diseases is considered the greatest
challenge in modern viticulture, due to the complexity of the different pathosystems and the lack of reliable control strategies.
During the last >100 years, vineyards have been routinely treated with fungicides, insecticides, herbicides and fertilizers.
Recent research have demonstrated that such treatments and other anthropic intervention (e.g. domestication, grafting,
training) affect the plant-associated microbiome. These human-driven alterations, exacerbated by grapevines clonal
propagation process, are believed to have led to an imbalance in grapevines endophytic microbiome, which may be a key
explanation for the recent success of GTD-associated pathogens. The best candidates to investigate the composition of a
balanced wood microbiome, unaffected by anthropic activities, are wild populations of V. vinifera subsp. sylvestris (VVS).
In this multidisciplinary project, I will join the fields of plant pathology, epigenetics and microbial ecology to (1) unravel the endophytic
microbiome diversity of wild populations of VVS, using DNA metabarcoding and in vitro isolation techniques; (2) re-introduce
endophytes isolated from VVS in cultivated grapevines, by means of a synthetic microbiome transfer; (3) assess the efficacy
of the synthetic microbiome in antagonizing GTD-associated pathogens, a next-generation approach to biological control.
The outcomes of this project will not only contribute to advance our understanding of grapevine endophytes ecology, but
have also the potential to provide a concrete solution to viticulture, for the benefit numerous European and non-European
countries.
Campo scientifico
Parole chiave
Programma(i)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Meccanismo di finanziamento
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinatore
33100 Udine
Italia