Description du projet
Une administration de médicaments télécommandée
Les exosomes sont des vésicules extracellulaires dérivées des cellules qui transportent des acides nucléiques, des protéines, des métabolites et des lipides. Ils sont impliqués dans la communication intercellulaire, et de nouvelles preuves mettent en évidence leur rôle dans la physiopathologie de nombreuses maladies. Le projet TheranoXome entend exploiter les exosomes pour procéder à une administration de médicaments guidée par imagerie. Financé par le programme Actions Marie Skłodowska-Curie, il utilisera des nanoparticules pour acheminer des médicaments chimiothérapeutiques à l’intérieur des exosomes, puis testera leurs propriétés et leur efficacité dans un modèle murin de cancer du sein. Il est important de noter que l’administration médicamenteuse et le devenir de ces exosomes seront contrôlés à l’aide de l’imagerie par résonance magnétique.
Objectif
The primary objective of this project is to engineer a non-immunogenic cell-membrane mimicking drug delivery vehicle for remote-controlled precision drug delivery. This will be achieved by encapsulating stimuli-responsive & contrast enhancing nanoparticles and drug cargo into endogenous exosomes to develop a therapeutic-cum-diagnostic exosomal system viz; TheranoXome for image-guided stimuli-responsive drug delivery. Such bio-mimetic TheranoXomes if realized will have wide applicability as a universal drug-delivery agent. The project entails three steps: (1) Synthesizing TheranoXomes by template assisted loading of chemotherapeutic drugs, light responsive thermoplasmonic gold nanorods and magnetic nanoparticles as magnetic resonance imaging agents into exosomes; (2) (Bio-)Physicochemical characterization of TheranoXomes including colloidal properties, biomimetics, immuno-compatibility, light-triggered drug release, magnetism (imaging and magnetic guided enrichment) and in vitro studies (2D and 3D cell cultures); (3) Application of TheranoXomes in vivo in a breast tumor bearing mice model. I (the ER) will prepare, characterize and test the materials and application proposed, with the support of a multidisciplinary hub of specialists. The prime training aim of TheranoXome is to provide me with a solid foundation in biomaterials, which will support my future career endeavours, both in the academia or industry, to be an independent researcher, a founder of a start-up company or to become key player in the biomaterials sector. I will be hosted by the Centre for Research in Biological Chemistry and Molecular Materials (CiQUS) of the University of Santiago de Compostela, a cutting-edge multidisciplinary research centre at the interface of chemistry, biomedicine and molecular materials. The training will be further strengthened by one secondment (6 months) focused on in vivo (drug delivery and imaging) studies (Health Research Institute of Santiago de Compostela - IDIS).
Champ scientifique
- natural sciencesbiological sciencescell biology
- medical and health sciencesclinical medicineoncology
- engineering and technologymedical engineeringdiagnostic imagingmagnetic resonance imaging
- engineering and technologynanotechnologynano-materials
- engineering and technologyindustrial biotechnologybiomaterials
Mots‑clés
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinateur
15782 Santiago De Compostela
Espagne