Project description
Gaining insight into ageing from bacterial transcriptional regulation
Sigma factors are the main constituents of transcription regulation in bacteria, playing a key role in gene expression regulation in response to environmental changes. They could provide insight into heterogeneity in ageing among individuals, going beyond bacteria and observed in diverse populations including humans. Scientific understanding of the molecular control of ageing via genetic and metabolic pathways does not fully explain this heterogeneity demonstrated by different ageing rates and patterns and even observed with fixed genetics and environment. With the support of the Marie Skłodowska-Curie Actions programme, the ASNet project will combine high-tech experimental techniques with mathematical modelling to elucidate the roles and mechanisms of four sigma factors in ageing processes of individual bacterial cells.
Objective
Ageing is controlled at the molecular level via genetic and metabolic pathways. Ageing rates and patterns vary substantially even with fixed genetics and environment. However, molecular mechanisms of ageing generating individual heterogeneity are poorly understood. Stochastic dynamics of gene expression can be a main source of the variation in cellular damage dynamics leading to individual heterogeneity in ageing. The sigma factor regulatory networks constitute the core part of the bacterial gene expression networks and therefore play important role in deciding the fate of bacterial cells. In this project, I focus on exploring the role and interplays of four major sigma factors rpoD, rpoS, rpoH, rpoN in individual E. coli cells. I plan to (1) obtain these sigma factors’ expression dynamics and the demographic fates at individual E. coli cells in microfluidic platforms under fluorescent microscope; (2) process and analyze big image data in an automated manner; (3) develop mathematical models to interpret expression and demographic signals for damage and ageing. I will combine the knowledge and methods from the disciplines of molecular biology, genetics, fluorescent microscopy and microfluidics, machine learning, population and evolutionary dynamics and mathematical modelling to shed light on the link from stochastic expression dynamics in sigma factor regulatory network towards individual variations observed in ageing fates.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2021-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
14195 BERLIN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.