Descrizione del progetto
Meccanismi genetico-molecolari della progenie ibrida disfunzionale nei parassiti agricoli
Alcuni processi, come le interazioni geniche incompatibili, l’accoppiamento con partner incompatibili o le incompatibilità citoplasmatiche mediate da parassiti batterici come Wolbachia, possono dare origine a una progenie ibrida disfunzionale nei parassiti agricoli. Sebbene queste progenie siano utilizzate in nuove strategie di controllo dei parassiti, esiste una lacuna nelle conoscenze relative ai meccanismi genetici molecolari che causano queste incompatibilità, che ostacola lo sviluppo di nuove innovazioni. Il progetto HYBRIPEST, finanziato dall’UE, si propone di studiare e scoprire i processi e i meccanismi responsabili di tali incompatibilità. Lo farà studiando un importante parassita che può portare a vari difetti dell’ibrido e che è influenzato da un kit genetico sviluppato di recente.
Obiettivo
Mating with an incompatible partner can result in dysfunctional hybrid offspring. Multiple processes cause such hybrid defects, including incompatible gene interactions and cytoplasmic incompatibilities that are mediated by bacterial parasites such as Wolbachia. Hybrid defects are strong reproductive barriers that drive speciation and are being harnessed by new pest control strategies to suppress harmful pest populations. Although pivotal to our understanding of these biological phenomena and applications, the molecular-genetic mechanisms that underpin these incompatibilities remain poorly understood. With HYBRIPEST, we will unravel all major mechanisms, and their interactions, that shape hybrid dysfunction within a system that recently became amenable to such a research endeavour. The system of Tetranychus mite species (important agricultural pests) is typified by various processes that culminate in different hybrid defects and benefits from a recently developed genetic toolkit. For the first time, we will dissect the molecular-genetic underpinnings of cytoplasmic incompatibility from the perspective of Wolbachia and host, an approach that will test biological theory and identify novel pest control opportunities. We will identify the molecular-genetic bases of incompatible gene interactions within and between species, shedding light on early speciation mechanisms. We will test for mechanistic convergence of different incompatibility loci and study how polygenic hybrid dysfunction is controlled. We will implement high-resolution genetic mapping techniques and comparative genomics to identify (in)compatibility loci. The functional importance of (in)compatibility loci to hybrid dysfunction will be further validated by recombinant expression, genome engineering, and other experimental advances. HYBRIPEST will bridge pertinent gaps in our mechanistic understanding of hybrid dysfunction, insights that will be translated into new theory and bio-applications.
Campo scientifico
Parole chiave
Programma(i)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Argomento(i)
Meccanismo di finanziamento
HORIZON-ERC - HORIZON ERC GrantsIstituzione ospitante
9000 Gent
Belgio