DYNASTY has successfully implemented a comprehensive programme of collaborative research and capacity-building activities, enabling FORTH to significantly enhance its expertise in the structural and opto-electronic characterization of two-dimensional (2D) materials. The project brought together complementary expertise from leading partners, combining atomic-scale structural analysis (EMAT, University of Antwerp) with advanced nonlinear optical imaging and spectroscopy (FORTH) and exciton dynamics (INSA Toulouse). This integration enabled the development of a multi-modal approach for the characterization of 2D materials, linking structural, optical and electronic properties with high precision. A major achievement of the project is the establishment and validation of advanced nonlinear imaging and spectroscopy capabilities at FORTH, allowing for high-resolution analysis of 2D materials and their heterostructures. These tools, combined with electron microscopy and exciton dynamics modelling, provide a powerful framework for understanding structure–property relationships, paving the way towards next-generation opto-valleytronic devices. The project also delivered a strong programme of training and knowledge exchange, including staff secondments, short visits, workshops and hands-on training activities. These actions enabled effective transfer of expertise, strengthening both technical skills and scientific collaboration among partners. In total, multiple exchanges and joint activities contributed to the development of new competencies in advanced characterization techniques and experimental methodologies. In parallel, DYNASTY fostered a dynamic collaborative environment, promoting continuous interaction between researchers. These exchanges facilitated the creation of new research ideas, strengthened international networks, and supported the development of future collaborations. Cross-disciplinary mentoring and interaction between researchers, technicians and administrative staff further contributed to building a cohesive and supportive research ecosystem. Overall, the project has significantly strengthened the research capacity of FORTH, established advanced experimental capabilities, and created a sustainable network for high-level collaboration in nanomaterials research across Europe.