Projektbeschreibung
Neuartige Metaboliten, die von Flechten in natürlicher Umgebung produziert werden, charakterisieren
Isolierte Mikroben sind nur ein sehr kleiner Teil der mikrobiellen Vielfalt in der natürlichen Umwelt. Ihre Erforschung hat zwar zu interessanten Verbindungen geführt, die dringend für neue antimikrobielle Mittel benötigt werden, aber die Entdeckung solcher Verbindungen ist in den vergangenen Jahrzehnten zurückgegangen. Im Rahmen des über die Marie-Skłodowska-Curie-Maßnahmen unterstützten Projekts FungalHetEx werden Flechten untersucht, eine mikrobielle Gemeinschaft, die Pilze, Grünalgen, Cyanobakterien und Bakterien umfasst. In einer kürzlich durchgeführten Studie wurden Hochdurchsatzverfahren und Multiomik-Analysen eingesetzt, um die anspruchsvolle Untersuchung von Flechten in ihrer natürlichen Umgebung zu erleichtern. Aufbauend auf diesem Wissen lautet das Ziel von FungalHetEx, synthetische Biotechnologie, Bioinformatik, Chemie und mikrobiologische Verfahren anzuwenden, um neuartige Metaboliten zu charakterisieren, die durch Symbiose in Flechtengemeinschaften entstehen.
Ziel
The increases in antimicrobial resistance and infections caused by microbes, such as mucormycosis (black fungus disease) on Covid-19 patients, highlight the urgent need for new antimicrobial drugs. Most drugs approved for clinical use are based or inspired by natural products. The recurrent re-discovery of compounds when studying isolated microbes, declined the research on natural products in the last decades. However, isolated microbes represent a small percentage of the true microbial diversity living in the environment. Lichens are a microbial community formed by different partners (fungi, green algae, cyanobacteria, bacteria) and their metabolites present diverse biological activities including antimicrobial. Furthermore, many of these molecules have vibrant colours and can be used as natural colorants. Due their slow growth and difficulties in the laboratory cultivation, little is known about natural products biosynthetic genes of the multiple partners living in symbiosis. The improvement in high-throughput technology and the current affordable prices for multi-omics analysis (meta-genomics, -transcriptomics and -bolomics), are allowing a deeper study on natural environmental samples. This project aims to use the knowledge obtained in a recent multi-omics study and apply synthetic biotechnology, bioinformatics, chemistry and microbiology techniques to study novel metabolites from lichen. Polyketide synthase biosynthetic gene clusters have been selected and they will be cloned, transformed and heterologously expressed using Aspergillus oryzae. The optimisation of the polyketides biosynthesis by the heterologous host will be performed and different fermentation conditions will be tested. Novel molecules will be chemically and biologically characterized. During FungalHetExt, I will study novel molecules produced under symbiosis using a multidisciplinary approach with the focus to discovery new molecules and improve my science communication and leadership skills.
Wissenschaftliches Gebiet
- natural sciencesbiological sciencesmicrobiologymycologyethnolichenology
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesbiological sciencessynthetic biology
- natural sciencesbiological sciencescell biologycell metabolism
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance
Schlüsselbegriffe
Programm/Programme
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenFinanzierungsplan
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsKoordinator
1011 JV AMSTERDAM
Niederlande