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Uncovering the regulatory landscape of Arabidopsis thaliana responses to cold temperatures

Project description

Understanding the mechanisms of plant adaptation to extreme cold

Although climate change is more often associated with global warming, it also includes the increasing frequency and intensity of cold temperatures. Understanding how plants react and adapt to periods of extreme cold – the regulatory networks and mechanisms – will be essential to global food security, yet little is currently known. However, evidence shows that low temperatures have a direct effect on chromatin accessibility, which in turn affects gene expression. With the support of the Marie Skłodowska-Curie Actions programme, the COOLCASE project will use the plant model system Arabidopsis thaliana to study the dynamics of the coding and non-coding transcriptome and changes in chromatin accessible sites in response to decreasing temperatures.

Objective

Plants, as sessile organisms, must constantly adapt to variable environmental conditions, among which temperature is of paramount importance in the context of climate change. Indeed, in future years, despite an expected rise in temperatures due to global warming, we can also expect an increase in the frequency of severe cold periods for which plants are not prepared. Therefore, understanding the molecular processes that allow plants to react and adapt to cold is essential for developing cold-resistant plants, which is ultimately indispensable for global food security. In recent decades many studies have embarked in addressing this issue. However, the global regulatory landscape underlying cold acclimation and adaption of plants to noisy and fluctuating natural cold conditions remain poorly investigated. Low temperatures are known to have a direct effect on chromatin accessibility which is directly linked to gene expression. Therefore, the aim of this project is to investigate the dynamics of the coding and non-coding Arabidopsis thaliana transcriptome, as well as changes in chromatin accessible sites, in response to regimes of decreasing temperatures. To this end, a comparative analysis of deep-coverage RNA-seq (transcriptome) and ATAC-seq (chromatin accessibility) will be performed on A. thaliana plants grown over weeks of fluctuating temperatures, mimicking natural cold conditions of autumn and early winter. The proposed experimental design will reveal novel coding genes, long non-coding RNAs (lncRNAs) and potential distal regulatory elements involved in Arabidopsis responses to cold. Thus, by combining interdisciplinary and mutually reinforcing approaches including (epi)genomics, (epi)genetics, transcriptomics and bioinformatics, the proposed project will significantly improve our understanding of the molecular mechanisms required for plant adaptation to cold temperatures likely delivering valuable tools for future breeding of cold resilient crop species.

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2022-PF-01

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Coordinator

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB
Net EU contribution

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€ 165 312,96
Address
PARC DE RECERCA DE L UAB CAMPUS UAB EDIFICI CRAG BELLATERRA
08193 CERDANYOLA DEL VALLES
Spain

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Region
Este Cataluña Barcelona
Activity type
Other
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Total cost

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