Impact of soil microbiodiversity on the development of wild mammal gut biota and immune function

Informazioni relative al progetto

SOIL2GUT

ID dell’accordo di sovvenzione: 101110279

DOI

10.3030/101110279

Progetto chiuso

Data della firma CE 14 Luglio 2023

Data di avvio 1 Novembre 2023

Data di completamento 31 Ottobre 2025

Finanziato da

• Marie Skłodowska-Curie Actions (MSCA)

Costo totale

Nessun dato

Contributo UE

€ 172 750,08

Coordinato da

FONDAZIONE EDMUND MACH
Italy

Periodic Reporting for period 1 - SOIL2GUT (Impact of soil microbiodiversity on the development of wild mammal gut biota and immune function)

Periodo di rendicontazione: 2023-11-01 al 2025-10-31

Sintesi del contesto e degli obiettivi generali del progetto

Healthy gut microbiota is essential for immune balance and overall well-being. Yet, modern urban lifestyles often limit contact with natural biodiversity, which may contribute to rising rates of immune-related conditions such as allergies and inflammatory diseases. The SOIL2GUT project set out to explore whether exposure to soil microbial communities during early development can influence gut microbiota and immune function.

Our objectives were twofold:
1. Determine if soil microbes affect not only gut bacteria but also gut fungi (mycobiota).
2. Assess whether the origin of soil—urban versus natural—leads to distinct impacts on gut microbiota and immune responses.

By addressing these gaps, the project aimed to provide evidence for nature-based strategies that promote health and resilience. The findings have implications for public health, urban planning, and biodiversity conservation, supporting EU priorities on sustainability and well-being.

Lavoro eseguito dall’inizio del progetto fino alla fine del periodo coperto dalla relazione e principali risultati finora ottenuti

We conducted a controlled biodiversity intervention using a wild-derived rodent model (bank vole). Animals were exposed for 4–5 weeks post-weaning to either sterile bedding (Control), soil from national parks (Park), or soil from urban greenspaces (Urban). Gut microbiota was analyzed using high-throughput sequencing of bacterial and fungal markers, and host immune responses were assessed through RNA sequencing of colon tissue.

Key achievements include:
-Soil exposure significantly altered gut microbiota composition and organization.
-Gut fungi showed a stronger response than bacteria, with many species acquired directly from soil.
-Urban soil exposure increased the abundance of potentially pathogenic bacterial genera (Allobaculum, Brachybacterium).
-Host gene expression revealed activation of innate immunity in animals exposed to urban soil, marked by upregulation of epithelial defense genes.
-Natural forest soils promoted immune tolerance, while urban soils triggered stronger immune activation.

All scientific objectives were met. All data and workflows have been archived in open repositories (ENA and Zenodo) to ensure transparency and reproducibility.

Progressi oltre lo stato dell’arte e potenziale impatto previsto (incluso l’impatto socioeconomico e le implicazioni sociali più ampie del progetto fino ad ora)

The project provides the first experimental evidence that soil exposure affects gut fungi as well as bacteria, highlighting fungi as a key but often overlooked component of biodiversity interventions. It also demonstrates that soil origin influences immune responses, with urban soils eliciting stronger activation of defense pathways. These findings advance the Biodiversity Hypothesis by showing that environmental microbial diversity impacts gut microbiota networks and host health in complex ways. Potential impacts include:
-Informing urban greenspace design to maximize health benefits while minimizing risks from pathogenic microbes.
-Supporting nature-based solutions in public health strategies to combat immune-mediated diseases.
-Opening new research avenues on the role of soil fungi in gut health and disease prevention.

Further uptake will require interdisciplinary studies linking soil management, microbiome science, and health policy, as well as guidelines for safe biodiversity interventions in schools, parks, and urban planning.

Outdoor activities in natural environments support gut microbiota development and immune health.